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PMID: 227917 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Effect of enzymes on rotavirus infectivity.

Journal of clinical microbiology ·Vol. 10 ·No. 1 ·1979-07-00 ·Pages 111-3

Barnett BB, Spendlove RS, Clark ML

Abstract

The infectivity of a bovine rotavirus was enhanced 140-, 8-, and 3-fold, respectively, by trypsin, protease, and lactase. Ficin, carboxypeptidases A and B, lysozyme, and beta-galactosidase had little effect on the infectivity. Chymotrypsin caused a threefold decrease in the infectivity. Trypsin acts directly on the rotavirus and not on the host cell.

MeSH Terms
Animals Cattle Cell Line Chymotrypsin/pharmacology Edetic Acid/pharmacology Glycoside Hydrolases/pharmacology Kidney Peptide Hydrolases/pharmacology RNA Viruses/drug effects Rotavirus/drug effects,growth & development,pathogenicity Trypsin/pharmacology Virulence Virus Replication/drug effects beta-Galactosidase/pharmacology
Chemicals
Edetic Acid Glycoside Hydrolases beta-Galactosidase Peptide Hydrolases Chymotrypsin Trypsin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Barnett B B
Spendlove R S
Clark M L
References (13)
13 references, click to expand
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  3. Production of high-titer bovine rotavirus with trypsin.
    J Clin Microbiol. 1979 Mar;9(3):413-7 PMID: 222801
  4. The effect of trypsin on the growth of rotavirus.
    J Gen Virol. 1978 Jul;40(1):213-8 PMID: 211180
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    J Clin Microbiol. 1977 Dec;6(6):610-7 PMID: 201663
  6. Cell culture propagation of porcine rotavirus (reovirus-like agent).
    Am J Vet Res. 1977 Nov;38(11):1765-8 PMID: 201198
  7. Further biochemical characterization, including the detection of surface glycoproteins, of human, calf, and simian rotaviruses.
    J Virol. 1977 Oct;24(1):91-8 PMID: 198589
  8. Is lactase the receptor and uncoating enzyme for infantile enteritis (rota) viruses?
    Lancet. 1976 Jun 26;1(7974):1387-8 PMID: 59021
  9. Characterization of two particle types of calf rotavirus.
    J Gen Virol. 1976 May;31(2):245-50 PMID: 180243
  10. Enhancement of reovirus infectivity by extracellular removal or alteration of the virus capsid by proteolytic enzymes.
    J Gen Virol. 1970 Aug;8(2):83-94 PMID: 4991060
  11. Infectivity assay of Reoviruses: comparison of immunofluorescent cell count and plaque methods.
    J Immunol. 1967 Jun;98(6):1301-8 PMID: 4961127
  12. Immunofluorescent cell assay of neonatal calf diarrhea virus.
    Can J Comp Med. 1975 Oct;39(4):462-5 PMID: 1100205
  13. Production in FL cells of infectious and potentially infectious reovirus.
    J Bacteriol. 1966 Oct;92(4):1036-40 PMID: 5926740
Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.10.1.111-113.1979
Published
1979-07-00
Pages
111-3
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC273105
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.10.1.111-113.1979 PMC Full Text View journal details
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