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PMID: 225640 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

A complementation analysis of mobilization deficient mutants of the plasmid ColE1.

Molecular & general genetics : MGG ·Vol. 172 ·No. 2 ·1979-05-04 ·Pages 211-9

Inselburg J, Ware P

Abstract

Hydroxylamine was used to induce mutants of the ColE1 derived plasmid pML2 that are inefficiently mobilized (Mob-) during conjugation by an Hfr donor. The ability of those mutants to be complemented by deletion mutants and Tn3 insertion mutants of ColE1 was examined. Three complementation groups were identified and localized on the ColE1 genetic map (Mob1, Mob2, and Mob3). One hydroxylamine mutant was not complemented by any mobilization deficient mutant but was complemented by mobilizable ColE1 mutants. Two hydroxylamine mutants were not complemented by any ColE1 derivatives. A mutant that had its relaxation nick site deleted had a markedly reduced mobilizability. The relationship between DNA relaxation nick site deleted had a markedly reduced mobilizability. THe relationship between DNA relaxation, replication and mobilization is considered.

MeSH Terms
Colicins DNA Topoisomerases, Type I/genetics DNA, Bacterial/genetics Escherichia coli/genetics Genetic Complementation Test Mutation Phenotype Plasmids
Chemicals
Colicins DNA, Bacterial DNA Topoisomerases, Type I
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Inselburg J
Ware P
References (24)
24 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00268285
Published
1979-05-04
Pages
211-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00268285
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