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PMID: 2254259 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

In vitro analysis of polypeptide requirements of multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600.

Journal of bacteriology ·Vol. 172 ·No. 12 ·1990-12-00 ·Pages 6834-40

Powlowski J, Shingler V

Abstract

An in vitro study of the multicomponent phenol hydroxylase from Pseudomonas sp. strain CF600 was performed. Phenol-stimulated oxygen uptake from crude extracts was strictly dependent on the addition of NAD(P)H and Fe2+ to assay mixtures. Five of six polypeptides required for growth on phenol were necessary for in vitro activity. One of the polypeptides was purified to homogeneity and found to be a flavin adenine dinucleotide containing iron-sulfur protein with significant sequence homology, at the amino terminus, to plant-type ferredoxins. This component, as in other oxygenase systems, probably functions to transfer electrons from NAD(P)H to the iron-requiring oxygenase component. Phenol hydroxylase from this organism is thus markedly different from bacterial flavoprotein monooxygenases commonly used for hydroxylation of other phenolic compounds, but bears a number of similarities to multicomponent oxygenase systems for unactivated compounds.

Related Genes
dmpK dmpL dmpM dmpN dmpO dmpP
MeSH Terms
Amino Acid Sequence Cloning, Molecular Macromolecular Substances Mixed Function Oxygenases/chemistry,isolation & purification Molecular Sequence Data NADH Dehydrogenase/metabolism Oxygenases/metabolism Phenol Phenols/metabolism Pseudomonas/enzymology Structure-Activity Relationship
Chemicals
Macromolecular Substances Phenols Phenol Mixed Function Oxygenases Oxygenases phenol 2-monooxygenase NADH Dehydrogenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Powlowski J
Unit for Applied Cell and Molecular Biology, University of Umeå, Sweden.
Shingler V
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42 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.12.6834-6840.1990
Published
1990-12-00
Pages
6834-40
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210800
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.172.12.6834-6840.1990 PMC Full Text View journal details
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