Isolation of phenol-degrading Bacillus stearothermophilus and partial characterization of the phenol hydroxylase.

Applied and environmental microbiology ·Vol. 55 ·No. 2 ·1989-02-00 ·Pages 500-2

Gurujeyalakshmi G, Oriel P

Abstract

Bacillus stearothermophilus BR219, isolated from river sediment, degraded phenol at levels to 15 mM at a rate of 0.85 mumol/h (4 x 10(6) cells). The solubilized phenol hydroxylase was NADH dependent, exhibited a 55 degrees C temperature optimum for activity, and was not inhibited by 0.5 mM phenol.

MeSH Terms

Geobacillus stearothermophilus/enzymology,growth & development,isolation & purification Kinetics Mixed Function Oxygenases/metabolism Phenols/metabolism Soil Microbiology

Chemicals

Phenols Mixed Function Oxygenases phenol 2-monooxygenase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Gurujeyalakshmi G

Department of Microbiology and Public Health, Michigan State University, East Lansing 48824-1101.

Oriel P

References (11)

11 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.55.2.500-502.1989

Published

1989-02-00

Pages

500-2

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC184139

Subset

External Links

PubMed source DOI: 10.1128/aem.55.2.500-502.1989 PMC Full Text View journal details

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Isolation of phenol-degrading Bacillus stearothermophilus and partial characterization of the phenol hydroxylase.

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