M.Smal is an N4-methylcytosine specific DNA-methylase.

Nucleic acids research ·Vol. 18 ·No. 22 ·1990-11-25 ·Pages 6607-9

Klimasauskas S, Steponaviciene D, Maneliene Z, Petrusyte M, Butkus V, Janulaitis A

Abstract

An enzymatic activity rendering DNA immune to the action of the Smal restriction endonuclease in the presence of S-adenosyl-L-methionine has been detected in Serratia marcescens Sb. This methylase, M.Smal, modifies the second cytosine residue of the substrate sequence CCCGGG yielding N4-methylcytosine.

MeSH Terms

Amino Acid Sequence Base Sequence Chromatography, High Pressure Liquid Cytosine/analogs & derivatives,metabolism DNA/chemistry Deoxyribonucleases, Type II Site-Specific/metabolism Hydrolysis Molecular Sequence Data Serratia marcescens/enzymology Substrate Specificity

Chemicals

N(4)-methylcytosine Cytosine DNA CCCGGG-specific type II deoxyribonucleases Deoxyribonucleases, Type II Site-Specific

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Klimasauskas S

Institute of Applied Enzymology, Vilnius, Lithuania, USSR.

Steponaviciene D

Maneliene Z

Petrusyte M

Butkus V

Janulaitis A

References (7)

7 references, click to expand

Detection of methylated sequences in eukaryotic DNA with the restriction endonucleases Smai and Xmai.
J Mol Biol. 1981 Jul 25;150(1):133-6 PMID: 6271972
N4-methylcytosine as a minor base in bacterial DNA.
J Bacteriol. 1987 Mar;169(3):939-43 PMID: 3029036
Cleavage of methylated CCCGGG sequences containing either N4-methylcytosine or 5-methylcytosine with MspI, HpaII, SmaI, XmaI and Cfr9I restriction endonucleases.
Nucleic Acids Res. 1987 Sep 11;15(17):7091-102 PMID: 2821492
Sequence motifs characteristic of DNA[cytosine-N4]methyltransferases: similarity to adenine and cytosine-C5 DNA-methylases.
Nucleic Acids Res. 1989 Dec 11;17(23):9823-32 PMID: 2690010
Restriction enzymes and their isoschizomers.
Nucleic Acids Res. 1989;17 Suppl:r347-87 PMID: 2541417
Cloning, characterization and heterologous expression of the SmaI restriction-modification system.
Nucleic Acids Res. 1989 Dec 11;17(23):9783-96 PMID: 2690008
Predictive motifs derived from cytosine methyltransferases.
Nucleic Acids Res. 1989 Apr 11;17(7):2421-35 PMID: 2717398

Article Info

Journal

Nucleic acids research

Abbr.

Nucleic Acids Res

ISSN

0305-1048

DOI

10.1093/nar/18.22.6607

Published

1990-11-25

Pages

6607-9

Language

English

Region

England

NLM ID

0411011

PMCID

PMC332617

Subset

External Links

PubMed source DOI: 10.1093/nar/18.22.6607 PMC Full Text View journal details

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