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PMID: 2247064 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The trypanosome leucine repeat gene in the variant surface glycoprotein expression site encodes a putative metal-binding domain and a region resembling protein-binding domains of yeast, Drosophila, and mammalian proteins.

Molecular and cellular biology ·Vol. 10 ·No. 12 ·1990-12-00 ·Pages 6436-44

Smiley BL, Stadnyk AW, Myler PJ, Stuart K

Abstract

We have identified a new variant surface glycoprotein expression site-associated gene (ESAG) in Trypanosoma brucei, the trypanosome leucine repeat (T-LR) gene. Like most other ESAGs, it is expressed in a life cycle stage-specific manner. The N-terminal 20% of the predicted T-LR protein resembles the metal-binding domains of nucleic acid-binding proteins. The remainder is composed of leucine-rich repeats that are characteristic of protein-binding domains found in a variety of other eucaryote proteins. This is the first report of leucine-rich repeats and potential nucleic acid-binding domains on the same protein. The T-LR gene is adjacent to ESAG 4, which has homology to the catalytic domain of adenylate cyclase. This is intriguing, since yeast adenylate cyclase has a leucine-rich repeat regulatory domain. The leucine-rich repeat and putative metal-binding domains suggest a possible regulatory role that may involve adenylate cyclase activity or nucleic acid binding.

MeSH Terms
Animals Base Sequence Binding Sites Cloning, Molecular Drosophila/genetics Genes Leucine Molecular Sequence Data Oligonucleotide Probes Repetitive Sequences, Nucleic Acid Restriction Mapping Saccharomyces cerevisiae/genetics Sequence Homology, Nucleic Acid Transcription, Genetic Trypanosoma brucei brucei/genetics,immunology Variant Surface Glycoproteins, Trypanosoma/genetics
Chemicals
Oligonucleotide Probes Variant Surface Glycoproteins, Trypanosoma Leucine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Smiley B L
Seattle Biomedical Research Institute, Washington 98109-1651.
Stadnyk A W
Myler P J
Stuart K
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.12.6436-6444.1990
Published
1990-12-00
Pages
6436-44
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC362920
Subset
IM
Grants
NIAID NIH HHS · AI 17375 · United States
Databases
GENBANK
M38528, M58701
External Links
PubMed source DOI: 10.1128/mcb.10.12.6436-6444.1990 PMC Full Text View journal details
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