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PMID: 2243381 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Generation of hybrid genes and proteins by vaccinia virus-mediated recombination: application to human immunodeficiency virus type 1 env.

Journal of virology ·Vol. 64 ·No. 12 ·1990-12-00 ·Pages 5948-57

Gritz L, Destree A, Cormier N, Day E, Stallard V, Caiazzo T, Mazzara G, Panicali D

Abstract

The ability of poxviruses to undergo intramolecular recombination within tandemly arranged homologous sequences can be used to generate chimeric genes and proteins. Genes containing regions of nucleotide homology will recombine to yield a single sequence composed of portions of both original genes. A recombinant virus containing two genes with a number of conserved regions will yield a population of recombinant viruses containing a spectrum of hybrid sequences derived by recombination between the original genes. This scheme has been used to generate hybrid human immunodeficiency virus type 1 env genes. Recombinant vaccinia viruses that contain two divergent env genes in tandem array have been constructed. In the absence of selective pressure to maintain both genes, recombination between conserved homologous regions in these genes generated a wide range of progeny, each of which expressed a novel variant polypeptide encoded by the newly created hybrid env gene. Poxvirus-mediated recombination may be applied to map type-specific epitopes, to create novel pharmaceuticals such as hybrid interferons, to study receptor-binding or enzyme substrate specificities, or to mimic the antigenic diversity found in numerous pathogens.

Related Genes
env
MeSH Terms
Animals Base Sequence Cell Line Chimera DNA, Viral/genetics,isolation & purification Gene Products, env/genetics Genes, Viral Genes, env HIV-1/genetics Humans Hybridization, Genetic Molecular Sequence Data Mutagenesis, Insertional Plasmids Recombination, Genetic Restriction Mapping Vaccinia virus/genetics
Chemicals
DNA, Viral Gene Products, env
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Gritz L
Applied bioTechnology, Inc, Cambridge, Massachusetts 02142.
Destree A
Cormier N
Day E
Stallard V
Caiazzo T
Mazzara G
Panicali D
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.12.5948-5957.1990
Published
1990-12-00
Pages
5948-57
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC248768
Subset
IM
Grants
PHS HHS · A126507 · United States
Databases
GENBANK
M12508, M38707, M38708
External Links
PubMed source DOI: 10.1128/JVI.64.12.5948-5957.1990 PMC Full Text View journal details
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