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PMID: 3260630 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

B- and T-lymphocyte responses to an immunodominant epitope of human immunodeficiency virus.

Journal of virology ·Vol. 62 ·No. 8 ·1988-08-00 ·Pages 2531-6

Schrier RD, Gnann JW, Langlois AJ, Shriver K, Nelson JA, Oldstone MB

Abstract

Using synthetic peptides, we characterized the B-lymphocyte (antibody) and T-lymphocyte (proliferation) responses to an immunodominant epitope of human immunodeficiency virus type 1 (HIV-1) located near the amino-terminal end of the transmembrane glycoprotein (env amino acids 598 to 609). Both immunoglobulin M (IgM) and IgG antibodies against this epitope appeared early after primary infection with HIV-1. In an animal model, the IgG response to a synthetic peptide derived from this sequence was T-helper-cell dependent, whereas the IgM response was T-cell independent. In addition, antibody generated by immunization with this peptide had HIV-1-neutralizing activity. Greater than 99% (201 of 203) of patients infected with HIV-1 generated antibody to this peptide in vivo; however, only 24% (7 of 29) had T cells that proliferated in response to this peptide in vitro. These observations suggest that different HIV-1 gp41 epitopes elicit B-cell and T-cell immune responses.

MeSH Terms
Animals Antibody Formation Antigens, Viral/immunology B-Lymphocytes/immunology HIV/immunology HIV Seropositivity/immunology Humans Lymphocyte Activation Mice Neutralization Tests Peptide Fragments/immunology T-Lymphocytes/immunology Time Factors Viral Envelope Proteins/immunology
Chemicals
Antigens, Viral Peptide Fragments Viral Envelope Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Schrier R D
Department of Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037.
Gnann J W
Langlois A J
Shriver K
Nelson J A
Oldstone M B
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.62.8.2531-2536.1988
Published
1988-08-00
Pages
2531-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC253681
Subset
IM
Grants
NIAID NIH HHS · AI-07007 · United States
NINDS NIH HHS · NS-12428 · United States
NINDS NIH HHS · T32-NS07078 · United States
External Links
PubMed source DOI: 10.1128/JVI.62.8.2531-2536.1988 PMC Full Text View journal details
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