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PMID: 2236034 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Beta-globin locus activation regions: conservation of organization, structure, and function.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 21 ·1990-11-00 ·Pages 8207-11

Li QL, Zhou B, Powers P, Enver T, Stamatoyannopoulos G

Abstract

The human beta-globin locus activation region (LAR) comprises four erythroid-specific DNase I hypersensitive sites (I-IV) thought to be largely responsible for activating the beta-globin domain and facilitating high-level erythroid-specific globin gene expression. We identified the goat beta-globin LAR, determined 10.2 kilobases of its sequence, and demonstrated its function in transgenic mice. The human and goat LARs share 6.5 kilobases of homologous sequences that are as highly conserved as the epsilon-globin gene promoters. Furthermore, the overall spatial organization of the two LARs has been conserved. These results suggest that the functionally relevant regions of the LAR are large and that in addition to their primary structure, the spatial relationship of the conserved elements is important for LAR function.

MeSH Terms
Animals Base Sequence Gene Expression Regulation Genes, Regulator Globins/genetics Goats Humans Mice Mice, Transgenic Molecular Sequence Data Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
Globins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Li Q L
Shanghai Institute of Biochemistry, Chinese Academy of Sciences.
Zhou B
Powers P
Enver T
Stamatoyannopoulos G
References (28)
28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.21.8207
Published
1990-11-00
Pages
8207-11
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54924
Subset
IM
Grants
NIDDK NIH HHS · DK 31232 · United States
NHLBI NIH HHS · HL 20899 · United States
Databases
GENBANK
M37648, M57436
External Links
PubMed source DOI: 10.1073/pnas.87.21.8207 PMC Full Text View journal details
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