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PMID: 2233679 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Colicin M is only bactericidal when provided from outside the cell.

Molecular & general genetics : MGG ·Vol. 222 ·No. 1 ·1990-06-00 ·Pages 37-40

Harkness RE, Braun V

Abstract

The colicin M structural gene, cma, was subcloned in a vector which allowed temperature-inducible control of its expression. Induction of expression of cma in colicin M uptake proficient strains was lethal for the host cell when the colicin M immunity protein was not present. In liquid culture cells lysed, and no colonies were formed on solid media. These effects were not observed in mutants defective in the colicin receptor (FhuA) or uptake functions (TonB, TolM), nor in wild-type cells treated with trypsin prior to induction of cma expression. It was concluded that cytoplasmic colicin M is not toxic for the producing cell. To exert a lethal effect the colicin has to enter the cell from outside. Cells expressing cma released small amounts of colicin M.

MeSH Terms
Bacterial Proteins/genetics,metabolism Bacteriolysis Cloning, Molecular Colicins/metabolism,toxicity Cytoplasm/metabolism Electrophoresis, Polyacrylamide Gel Escherichia coli/genetics,growth & development,metabolism Escherichia coli Proteins Gene Expression Regulation, Bacterial Genes, Bacterial Receptors, Cell Surface Receptors, Immunologic/metabolism Temperature
Chemicals
Bacterial Proteins Colicins Escherichia coli Proteins Receptors, Cell Surface Receptors, Immunologic cmI protein, E coli colicin immunity proteins colicin receptor, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Harkness R E
Mikrobiologie II, Universität Tübingen, Federal Republic of Germany.
Braun V
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35 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00283020
Published
1990-06-00
Pages
37-40
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00283020
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