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PMID: 22330896 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

TaNAC2, a NAC-type wheat transcription factor conferring enhanced multiple abiotic stress tolerances in Arabidopsis.

Journal of experimental botany ·Vol. 63 ·No. 8 ·2012-05-00 ·Pages 2933-46

Mao X, Zhang H, Qian X, Li A, Zhao G, Jing R

Abstract

Environmental stresses such as drought, salinity, and cold are major factors that significantly limit agricultural productivity. NAC transcription factors play essential roles in response to various abiotic stresses. However, the paucity of wheat NAC members functionally characterized to date does not match the importance of this plant as a world staple crop. Here, the function of TaNAC2 was characterized in Arabidopsis thaliana. A fragment of TaNAC2 was obtained from suppression subtractive cDNA libraries of wheat treated with polyethylene glycol, and its full-length cDNA was obtained by searching a full-length wheat cDNA library. Gene expression profiles indicated that TaNAC2 was involved in response to drought, salt, cold, and abscisic acid treatment. To test its function, transgenic Arabidopsis lines overexpressing TaNAC2-GFP controlled by the cauliflower mosaic virus 35S promoter were generated. Overexpression of TaNAC2 resulted in enhanced tolerances to drought, salt, and freezing stresses in Arabidopsis, which were simultaneously demonstrated by enhanced expression of abiotic stress-response genes and several physiological indices. Therefore, TaNAC2 has potential for utilization in transgenic breeding to improve abiotic stress tolerances in crops.

MeSH Terms
Adaptation, Physiological/genetics Amino Acid Sequence Arabidopsis/genetics,physiology Chromosomes, Plant/genetics Gene Expression Regulation, Plant Genes, Plant/genetics Molecular Sequence Data Osmotic Pressure Phylogeny Plant Proteins/chemistry,genetics,metabolism Plant Roots/anatomy & histology Plants, Genetically Modified Protein Transport Quantitative Trait, Heritable RNA, Messenger/genetics,metabolism Sequence Alignment Stress, Physiological/genetics Subcellular Fractions/metabolism Transcription Factors/chemistry,genetics,metabolism Triticum/metabolism
Chemicals
Plant Proteins RNA, Messenger Transcription Factors
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mao Xinguo
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Zhang Hongying
Qian Xueya
Li Ang
Zhao Guangyao
Jing Ruilian
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Article Info
Journal
Journal of experimental botany
Abbr.
J Exp Bot
ISSN
1460-2431
DOI
10.1093/jxb/err462
Published
2012-05-00
Epub
2012-00-13
Pages
2933-46
Language
English
Region
England
NLM ID
9882906
PMCID
PMC3350912
Subset
IM
External Links
PubMed source DOI: 10.1093/jxb/err462 PMC Full Text View journal details
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