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PMID: 20022921 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

TaSnRK2.4, an SNF1-type serine/threonine protein kinase of wheat (Triticum aestivum L.), confers enhanced multistress tolerance in Arabidopsis.

Journal of experimental botany ·Vol. 61 ·No. 3 ·2010-03-00 ·Pages 683-96

Mao X, Zhang H, Tian S, Chang X, Jing R

Abstract

Osmotic stresses such as drought, salinity, and cold are major environmental factors that limit agricultural productivity worldwide. Protein phosphorylation/dephosphorylation are major signalling events induced by osmotic stress in higher plants. Sucrose non-fermenting 1-related protein kinase2 family members play essential roles in response to hyperosmotic stresses in Arabidopsis, rice, and maize. In this study, the function of TaSnRK2.4 in drought, salt, and freezing stresses in Arabidopsis was characterized. A translational fusion protein of TaSnRK2.4 with green fluorescent protein showed subcellular localization in the cell membrane, cytoplasm, and nucleus. To examine the role of TaSnRK2.4 under various environmental stresses, transgenic Arabidopsis plants overexpressing wheat TaSnRK2.4 under control of the cauliflower mosaic virus 35S promoter were generated. Overexpression of TaSnRK2.4 resulted in delayed seedling establishment, longer primary roots, and higher yield under normal growing conditions. Transgenic Arabidopsis overexpressing TaSnRK2.4 had enhanced tolerance to drought, salt, and freezing stresses, which were simultaneously supported by physiological results, including decreased rate of water loss, enhanced higher relative water content, strengthened cell membrane stability, improved photosynthesis potential, and significantly increased osmotic potential. The results show that TaSnRK2.4 is involved in the regulation of enhanced osmotic potential, growth, and development under both normal and stress conditions, and imply that TaSnRK2.4 is a multifunctional regulatory factor in Arabidopsis. Since the overexpression of TaSnRK2.4 can significantly strengthen tolerance to drought, salt, and freezing stresses and does not retard the growth of transgenic Arabidopsis plants under well-watered conditions, TaSnRK2.4 could be utilized in transgenic breeding to improve abiotic stresses in crops.

MeSH Terms
Adaptation, Physiological/drug effects Amino Acid Sequence Arabidopsis/cytology,drug effects,genetics,growth & development Cell Membrane/drug effects Droughts Freezing Gene Expression Profiling Gene Expression Regulation, Developmental/drug effects Gene Expression Regulation, Plant/drug effects Molecular Sequence Data Osmosis/drug effects Photosynthesis/drug effects Phylogeny Plant Proteins/chemistry,genetics,metabolism Plants, Genetically Modified Protein Serine-Threonine Kinases/chemistry,genetics,metabolism Protein Transport/drug effects Sequence Alignment Sodium Chloride/pharmacology Stress, Physiological/drug effects Subcellular Fractions/drug effects,enzymology Triticum/drug effects,enzymology,genetics Water/metabolism
Chemicals
Plant Proteins Water Sodium Chloride Protein Serine-Threonine Kinases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Mao Xinguo
The National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Zhang Hongying
Tian Shanjun
Chang Xiaoping
Jing Ruilian
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Article Info
Journal
Journal of experimental botany
Abbr.
J Exp Bot
ISSN
1460-2431
DOI
10.1093/jxb/erp331
Published
2010-03-00
Epub
2009-00-18
Pages
683-96
Language
English
Region
England
NLM ID
9882906
PMCID
PMC2814103
Subset
IM
Databases
GENBANK
GQ384359
External Links
PubMed source DOI: 10.1093/jxb/erp331 PMC Full Text View journal details
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