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PMID: 22247174 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of the herboxidiene biosynthetic gene cluster in Streptomyces chromofuscus ATCC 49982.

Applied and environmental microbiology ·Vol. 78 ·No. 6 ·2012-03-00 ·Pages 2034-8

Shao L, Zi J, Zeng J, Zhan J

Abstract

The 53-kb biosynthetic gene cluster for the novel anticholesterol natural product herboxidiene was identified in Streptomyces chromofuscus ATCC 49982 by genome sequencing and gene inactivation. In addition to herboxidiene, a biosynthetic intermediate, 18-deoxy-herboxidiene, was also isolated from the fermentation broth of S. chromofuscus ATCC 49982 as a minor metabolite.

MeSH Terms
Biosynthetic Pathways/genetics Culture Media/chemistry DNA, Bacterial/chemistry,genetics Fatty Alcohols/metabolism Gene Knockout Techniques Molecular Sequence Data Multigene Family Pyrans/metabolism Sequence Analysis, DNA Streptomyces/genetics,metabolism
Chemicals
Culture Media DNA, Bacterial Fatty Alcohols Pyrans herboxidiene
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shao Lei
Department of Biological Engineering, Utah State University, Logan, Utah, USA.
Zi Jiachen
Zeng Jia
Zhan Jixun
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24 references, click to expand
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Article Info
Journal
Applied and environmental microbiology
Abbr.
Appl Environ Microbiol
ISSN
1098-5336
DOI
10.1128/AEM.06904-11
Published
2012-03-00
Epub
2012-00-13
Pages
2034-8
Language
English
Region
United States
NLM ID
7605801
PMCID
PMC3298124
Subset
IM
Databases
GENBANK
JN671974
External Links
PubMed source DOI: 10.1128/AEM.06904-11 PMC Full Text View journal details
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