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PMID: 22194609 Published · ppublish English Journal Article

Epithelial protein lost in neoplasm (EPLIN) interacts with α-catenin and actin filaments in endothelial cells and stabilizes vascular capillary network in vitro.

The Journal of biological chemistry ·Vol. 287 ·No. 10 ·2012-03-02 ·Pages 7556-72

Chervin-Pétinot A, Courçon M, Almagro S, Nicolas A, Grichine A, Grunwald D, Prandini MH, Huber P, Gulino-Debrac D

Abstract

Adherens junctions are required for vascular endothelium integrity. These structures are formed by the clustering of the homophilic adhesive protein VE-cadherin, which recruits intracellular partners, such as β- and α-catenins, vinculin, and actin filaments. The dogma according to which α-catenin bridges cadherin·β-catenin complexes to the actin cytoskeleton has been challenged during the past few years, and the link between the VE-cadherin·catenin complex and the actin cytoskeleton remains unclear. Recently, epithelial protein lost in neoplasm (EPLIN) has been proposed as a possible bond between the E-cadherin·catenin complex and actin in epithelial cells. Herein, we show that EPLIN is expressed at similar levels in endothelial and epithelial cells and is located at interendothelial junctions in confluent cells. Co-immunoprecipitation and GST pulldown experiments provided evidence that EPLIN interacts directly with α-catenin and tethers the VE-cadherin·catenin complex to the actin cytoskeleton. In the absence of EPLIN, vinculin was delocalized from the junctions. Furthermore, suppression of actomyosin tension using blebbistatin triggered a similar vinculin delocalization from the junctions. In a Matrigel assay, EPLIN-depleted endothelial cells exhibited a reduced capacity to form pseudocapillary networks because of numerous breakage events. In conclusion, we propose a model in which EPLIN establishes a link between the cadherin·catenin complex and actin that is independent of actomyosin tension. This link acts as a mechanotransmitter, allowing vinculin binding to α-catenin and formation of a secondary molecular bond between the adherens complex and the cytoskeleton through vinculin. In addition, we provide evidence that the EPLIN clutch is necessary for stabilization of capillary structures in an angiogenesis model.

MeSH Terms
Actin Cytoskeleton/genetics,metabolism Adherens Junctions/genetics,metabolism Animals Antigens, CD/genetics,metabolism Caco-2 Cells Cadherins/genetics,metabolism Capillaries/cytology,metabolism Cytoskeletal Proteins/genetics,metabolism Dogs Endothelial Cells/cytology,metabolism Epithelial Cells/cytology,metabolism Humans Mechanotransduction, Cellular/physiology Models, Biological Multiprotein Complexes/genetics,metabolism Neovascularization, Physiologic/physiology Vinculin/genetics,metabolism alpha Catenin/genetics,metabolism
Chemicals
Antigens, CD Cadherins Cytoskeletal Proteins LIMA1 protein, human Multiprotein Complexes VCL protein, human alpha Catenin cadherin 5 Vinculin
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Chervin-Pétinot Adeline
INSERM U882, F-38054 Grenoble, France.
Courçon Marie
Almagro Sébastien
Nicolas Alice
Grichine Alexei
Grunwald Didier
Prandini Marie-Hélène
Huber Philippe
Gulino-Debrac Danielle
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
1083-351X
Published
2012-03-02
Epub
2011-00-22
Pages
7556-72
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC3293581
Subset
IM
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