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PMID: 22178397 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Activator-induced spread of poly(ADP-ribose) polymerase promotes nucleosome loss at Hsp70.

Molecular cell ·Vol. 45 ·No. 1 ·2012-01-13 ·Pages 64-74

Petesch SJ, Lis JT

Abstract

Eukaryotic cells possess many transcriptionally regulated mechanisms to alleviate the nucleosome barrier. Dramatic changes to the chromatin structure of Drosophila melanogaster Hsp70 gene loci are dependent on the transcriptional activator, heat shock factor (HSF), and poly(ADP-ribose) polymerase (PARP). Here, we find that PARP is associated with the 5' end of Hsp70, and its enzymatic activity is rapidly induced by heat shock. This activation causes PARP to redistribute throughout Hsp70 loci and Poly(ADP-ribose) to concurrently accumulate in the wake of PARP's spread. HSF is necessary for both the activation of PARP's enzymatic activity and its redistribution. Upon heat shock, HSF triggers these PARP activities mechanistically by directing Tip60 acetylation of histone H2A lysine 5 at the 5' end of Hsp70, where inactive PARP resides before heat shock. This acetylation is critical for the activation and spread of PARP as well as for the rapid nucleosome loss over the Hsp70 loci.

MeSH Terms
Acetylation Animals Drosophila Proteins/metabolism,physiology Drosophila melanogaster/enzymology,genetics HSP70 Heat-Shock Proteins/genetics,metabolism Heat-Shock Response Histone Acetyltransferases/metabolism Histone Deacetylases/metabolism,physiology Histones/metabolism Nucleosomes/genetics,metabolism Poly(ADP-ribose) Polymerases/metabolism,physiology
Chemicals
Drosophila Proteins HSP70 Heat-Shock Proteins Histones Nucleosomes Histone Acetyltransferases Tip60 protein, Drosophila Poly(ADP-ribose) Polymerases Histone Deacetylases histone deacetylase 3
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Petesch Steven J
Department of Molecular Biology and Genetics, Cornell University, Ithaca, NY 14853, USA.
Lis John T
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Article Info
Journal
Molecular cell
Abbr.
Mol Cell
ISSN
1097-4164
DOI
10.1016/j.molcel.2011.11.015
Published
2012-01-13
Epub
2011-00-15
Pages
64-74
Language
English
Region
United States
NLM ID
9802571
PMCID
PMC3473076
Subset
IM
Grants
NIGMS NIH HHS · GM25232 · United States
NIGMS NIH HHS · R01 GM025232-35 · United States
NIGMS NIH HHS · T32-GM70723 · United States
NIGMS NIH HHS · R37 GM025232 · United States
NIGMS NIH HHS · R01 GM025232-34 · United States
NIGMS NIH HHS · R01 GM025232 · United States
External Links
PubMed source DOI: 10.1016/j.molcel.2011.11.015 PMC Full Text View journal details
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