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PMID: 221674 Published · ppublish English Journal Article

Cell-free translation of simian virus 40 16S and 19S L-strand-specific mRNA classes to simian virus 40 major VP-1 and minor VP-2 and VP-3 capsid proteins.

Journal of virology ·Vol. 29 ·No. 3 ·1979-03-00 ·Pages 1204-12

Prives CL, Shure H

Abstract

Simian virus 40 capsid proteins VP-1, VP-2, and VP-3 have been synthesized in wheat germ and reticulocyte cell-free systems in response to either poly(A)-containing mRNA from the cytoplasm of infected cells or viral RNA purified by hybridization to simian virus 40 DNA linked to Sepharose. All three viral polypeptides synthesized in vitro are specifically immunoprecipitated with anti-simian virus 40 capsid serum. VP-2 and VP-3 are related by tryptic peptide mapping to each other but not to VP-1. The most abundant class of L-strand-specific viral mRNA, the 16S species, codes for the major capsid protein. The relatively minor 19S class directs the cell-free synthesis of VP-1, VP-2, and VP-3. Whether the 19S RNA represents more than one distinct species of mRNA is not yet clear. VP-1 mRNA can be isolated from the cytoplasm, detergent-washed nuclei, and the nuclear wash fraction. The mRNA from the nuclear wash fraction is enriched for VP-2 mRNA when compared to other viral or cellular polypeptides.

MeSH Terms
Capsid/biosynthesis Cell Line Cell Nucleus/analysis Cell-Free System Cytoplasm/analysis Peptide Biosynthesis Protein Biosynthesis RNA, Messenger/isolation & purification,metabolism RNA, Viral/isolation & purification,metabolism Simian virus 40/genetics,metabolism Viral Proteins/biosynthesis
Chemicals
RNA, Messenger RNA, Viral Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Prives C L
Shure H
References (32)
32 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.29.3.1204-1212.1979
Published
1979-03-00
Pages
1204-12
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC353281
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.29.3.1204-1212.1979 PMC Full Text View journal details
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