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PMID: 22084416 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Expression and characterization of the bacterial mechanosensitive channel MscS in Xenopus laevis oocytes.

The Journal of general physiology ·Vol. 138 ·No. 6 ·2011-12-00 ·Pages 641-9

Maksaev G, Haswell ES

Abstract

We have successfully expressed and characterized mechanosensitive channel of small conductance (MscS) from Escherichia coli in oocytes of the African clawed frog, Xenopus laevis. MscS expressed in oocytes has the same single-channel conductance and voltage dependence as the channel in its native environment. Two hallmarks of MscS activity, the presence of conducting substates at high potentials and reversible adaptation to a sustained stimulus, are also exhibited by oocyte-expressed MscS. In addition to its ease of use, the oocyte system allows the user to work with relatively large patches, which could be an advantage for the visualization of membrane deformation. Furthermore, MscS can now be compared directly to its eukaryotic homologues or to other mechanosensitive channels that are not easily studied in E. coli.

MeSH Terms
Animals Escherichia coli Proteins/genetics,metabolism Female Ion Channel Gating Ion Channels/genetics,metabolism Membrane Potentials Microscopy, Confocal Oocytes/metabolism Xenopus laevis
Chemicals
Escherichia coli Proteins Ion Channels MscS protein, E coli
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Maksaev Grigory
Department of Biology, Washington University in Saint Louis, Saint Louis, MO 63130, USA.
Haswell Elizabeth S
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Article Info
Journal
The Journal of general physiology
Abbr.
J Gen Physiol
ISSN
1540-7748
DOI
10.1085/jgp.201110723
Published
2011-12-00
Epub
2011-00-14
Pages
641-9
Language
English
Region
United States
NLM ID
2985110R
PMCID
PMC3226970
Subset
IM
Grants
NIGMS NIH HHS · R01 GM084211 · United States
NIGMS NIH HHS · R01GM084211-01 · United States
External Links
PubMed source DOI: 10.1085/jgp.201110723 PMC Full Text View journal details
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