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PMID: 220614 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular reorganization of lipid bilayers by complement: a possible mechanism for membranolysis.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 76 ·No. 3 ·1979-03-00 ·Pages 1410-4

Esser AF, Kolb WP, Podack ER, Müller-Eberhard HJ

Abstract

The interaction between the membrane attack complex (MAC) of complement and flat lipid bilayers was investigated. Using spin-labeled derivatives of phospholipids and cholesterol and electron paramagnetic resonance spectroscopy, we measured the penetration of the MAC into bilayers and its influence on the order of bilayers. The MAC precursor components C5b--6, C7, C8, and C9 did not exert any measurable influence on lipid membranes. Functional C5b--7 was shown to interact strongly with the bilayer surface without deep penetration into the bilayer. Formation of C5b--8 and especially C5b--9 caused a marked change in the anisotropy of spectra from probes located within the hydrocarbon phase. The spectral changes are not caused by changes in probe rotation and, in the case of the cholesterol probes, are not due to direct probe--protein interactions. For these reasons we interpret the spectral changes to be the result of reorientation of ordered bilayer lipids effected by strong binding of phospholipids to MAC proteins.

MeSH Terms
Cholesterol Complement System Proteins Electron Spin Resonance Spectroscopy Membranes, Artificial Models, Biological Molecular Conformation Phosphatidylcholines Protein Binding Protein Conformation Spin Labels
Chemicals
Membranes, Artificial Phosphatidylcholines Spin Labels Complement System Proteins Cholesterol
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Esser A F
Kolb W P
Podack E R
Müller-Eberhard H J
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27 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.76.3.1410
Published
1979-03-00
Pages
1410-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC383261
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.76.3.1410 PMC Full Text View journal details
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