Electron spin resonance studies on interaction of complement proteins with erythrocyte membranes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 75 ·No. 10 ·1978-10-00 ·Pages 4930-4

Dahl CE, Levine RP

Abstract

Sheep erythrocytes have been spin labeled with 5-, 12-, and 16-nitroxystearic acid in order to investigate complement-induced changes in the physical state of the lipid bilayer. Formation of osmotic lesions in the membrane causes an increase in the fluidity of the membrane which overcomes the decrease in membrane fluidity caused by the interaction of the complement proteins. A decrease in membrane fluidity is observed only when complement-lysed membranes are resealed or when complement proteins react with isosmolar ghosts that do not undergo osmotic lysis. The decrease in bulk fluidity of the membrane is first observed when C8 binds to the membranes bearing C5b67 and is enhanced upon the subsequent binding of C9. The decrease in membrane fluidity shown by the electron spin resonance spectra of spin-labeled fatty acids suggests that certain of the complement proteins penetrate the membrane and interact with hydrophobic regions of the lipid bilayer.

MeSH Terms

Animals Complement C5/metabolism Complement C8/metabolism Complement C9/metabolism Complement System Proteins/metabolism Electron Spin Resonance Spectroscopy Erythrocyte Membrane/physiology Erythrocytes/physiology In Vitro Techniques Membrane Fluidity Sheep

Chemicals

Complement C5 Complement C8 Complement C9 Complement System Proteins

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Dahl C E

Levine R P

References (29)

29 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.75.10.4930

Published

1978-10-00

Pages

4930-4

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC336235

Subset

External Links

PubMed source DOI: 10.1073/pnas.75.10.4930 PMC Full Text View journal details

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Electron spin resonance studies on interaction of complement proteins with erythrocyte membranes.

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