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PMID: 22004760 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

GB1 is not a two-state folder: identification and characterization of an on-pathway intermediate.

Biophysical journal ·Vol. 101 ·No. 8 ·2011-10-19 ·Pages 2053-60

Morrone A, Giri R, Toofanny RD, Travaglini-Allocatelli C, Brunori M, Daggett V, Gianni S

Abstract

The folding pathway of the small α/β protein GB1 has been extensively studied during the past two decades using both theoretical and experimental approaches. These studies provided a consensus view that the protein folds in a two-state manner. Here, we reassessed the folding of GB1, both by experiments and simulations, and detected the presence of an on-pathway intermediate. This intermediate has eluded earlier experimental characterization and is distinct from the collapsed state previously identified using ultrarapid mixing. Failure to identify the presence of an intermediate affects some of the conclusions that have been drawn for GB1, a popular model for protein folding studies.

MeSH Terms
Bacterial Proteins/chemistry Hydrogen-Ion Concentration Kinetics Molecular Dynamics Simulation Protein Conformation Protein Folding Thermodynamics
Chemicals
Bacterial Proteins IgG Fc-binding protein, Streptococcus
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Morrone Angela
Istituto Pasteur-Fondazione Cenci Bolognetti and Istituto di Biologia e Patologia Molecolari del CNR, Dipartimento di Scienze Biochimiche "A. Rossi Fanelli", Università di Roma "La Sapienza", Rome, Italy.
Giri Rajanish
Toofanny Rudesh D
Travaglini-Allocatelli Carlo
Brunori Maurizio
Daggett Valerie
Gianni Stefano
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Article Info
Journal
Biophysical journal
Abbr.
Biophys J
ISSN
1542-0086
DOI
10.1016/j.bpj.2011.09.013
Published
2011-10-19
Pages
2053-60
Language
English
Region
United States
NLM ID
0370626
PMCID
PMC3192981
Subset
IM
Grants
NIGMS NIH HHS · R01 GM050789 · United States
NIGMS NIH HHS · R29 GM050789 · United States
NIGMS NIH HHS · GM50789 · United States
External Links
PubMed source DOI: 10.1016/j.bpj.2011.09.013 PMC Full Text View journal details
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