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PMID: 2199817 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Repair of alkylation damage in Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 221 ·No. 3 ·1990-05-00 ·Pages 353-7

Goth-Goldstein R, Johnson PL

Abstract

Repair of methylated bases in Saccharomyces cerevisiae was measured by two methods: in vitro in cell extracts, and in vivo, by determining the loss of methylated bases from yeast DNA after treatment of stationary cultures with [3H]-N-methyl-N'-nitro-N-nitrosoguanidine. Whereas no repair activity could be detected by the in vitro method, the methylated bases were removed in vivo very efficiently. These contradictory results of in vitro and in vivo repair measurements suggest that either the repair enzymes of yeast are sufficiently different from those of bacteria and mammalian cells that they are not active in the in vitro assay, or that methylated bases are repaired in yeast by a different pathway.

MeSH Terms
Alkylation DNA Damage DNA Repair DNA, Fungal/drug effects Nitrosamines/pharmacology Saccharomyces cerevisiae/drug effects,genetics
Chemicals
DNA, Fungal Nitrosamines 3-(methylnitrosamino)propionitrile
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Goth-Goldstein R
Cell and Molecular Biology Division, Lawrence Berkeley Laboratory, Berkeley, CA 94720.
Johnson P L
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22 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00259399
Published
1990-05-00
Pages
353-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
FDA HHS · 2S07 BR05918-04 · United States
NIEHS NIH HHS · ES03603 · United States
External Links
PubMed source DOI: 10.1007/BF00259399
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