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PMID: 2196441 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The cytokine response element of the rat alpha 1-acid glycoprotein gene is a complex of several interacting regulatory sequences.

Molecular and cellular biology ·Vol. 10 ·No. 8 ·1990-08-00 ·Pages 3965-78

Won KA, Baumann H

Abstract

Expression of the rat alpha 1-acid glycoprotein gene is stimulated by interleukin-1 (IL-1) and interleukin-6 (IL-6) and is synergistically enhanced by the combination of the two. The distal regulatory element (DRE), a 142-base-pair (bp) sequence located 5 kilobase pairs upstream of the transcriptional start site, appears to be crucial for this cytokine response. The cytokine-specific regulatory sequences within the DRE have been identified by inserting individual DRE subregions, selected combinations of these, or a few linker mutated fragments into a plasmid containing an enhancerless simian virus 40 promoter linked to the chloramphenicol acetyltransferase gene. The regulatory activity was determined in transiently transfected human and rat hepatoma cells. The IL-1 response region was confined to the 5'-most 62 bp of the DRE, and its function seemed to depend on at least two separate components. The same region was also responsive to phorbol ester treatment. The IL-6 regulatory function was dependent on a 54-bp sequence located within the 3' half of the DRE. When the IL-1 response region was recombined with the IL-6 regulatory region of the DRE or with IL-6 response elements of other plasma protein genes, a strong cooperative action by IL-1 and IL-6 was achieved. The functional DRE sequences were recognized by nuclear proteins extracted from rat liver and hepatoma cells. However, no cytokine-inducible binding activity was detectable, which suggests that transcriptional regulation through the DRE might be controlled by posttranslational modification of constitutively bound trans-acting factors.

MeSH Terms
Animals Base Sequence Biological Factors/pharmacology Cell Line Cytokines DNA-Binding Proteins/isolation & purification,metabolism Gene Expression Regulation/drug effects Genes/drug effects Humans Interleukin-1/pharmacology Interleukin-6/pharmacology Liver/metabolism Liver Neoplasms, Experimental/metabolism Molecular Sequence Data Orosomucoid/genetics Plasmids Rats Recombinant Proteins/pharmacology Regulatory Sequences, Nucleic Acid Sequence Homology, Nucleic Acid Transfection
Chemicals
Biological Factors Cytokines DNA-Binding Proteins Interleukin-1 Interleukin-6 Orosomucoid Recombinant Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Won K A
Department of Molecular and Cellular Biology, Roswell Park Cancer Institute, Buffalo, New York 14263.
Baumann H
References (67)
67 references, click to expand
  1. IL-6/BSF2 in normal and abnormal regulation of immune responses.
    Ann N Y Acad Sci. 1989;557:466-76; discussion 476-7 PMID: 2786703
  2. The human haptoglobin gene promoter: interleukin-6-responsive elements interact with a DNA-binding protein induced by interleukin-6.
    EMBO J. 1989 Apr;8(4):1145-51 PMID: 2787245
  3. Identification of cis-acting sequences responsible for phorbol ester induction of human serum amyloid A gene expression via a nuclear factor kappaB-like transcription factor.
    Mol Cell Biol. 1989 May;9(5):1908-16 PMID: 2747640
  4. Using mini-prep plasmid DNA for sequencing double stranded templates with Sequenase.
    Biotechniques. 1988 Jun;6(6):544-6, 549 PMID: 3273187
  5. Structure, hormonal regulation, and identification of the interleukin-6- and dexamethasone-responsive element of the rat haptoglobin gene.
    Mol Cell Biol. 1990 Apr;10(4):1573-83 PMID: 2320005
  6. A transplantable bile-secreting hepatocellular carcinoma in the rat.
    J Natl Cancer Inst. 1961 Apr;26:891-9 PMID: 13740982
  7. Cyclic AMP--an intracellular second messenger for interleukin 1.
    Proc Natl Acad Sci U S A. 1988 Nov;85(21):8201-5 PMID: 2847154
  8. Many transcription factors interact synergistically with steroid receptors.
    Science. 1988 Dec 9;242(4884):1418-20 PMID: 3201230
  9. IL-1 and TNF transmodulate epidermal growth factor receptors by a protein kinase C-independent mechanism.
    J Immunol. 1989 Jan 1;142(1):126-33 PMID: 2783320
  10. Interleukin-1 and interleukin-6 stimulate acute-phase protein production in primary mouse hepatocytes.
    J Leukoc Biol. 1989 Jan;45(1):55-61 PMID: 2463323
  11. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  12. Crossed immunoelectrophoresis.
    Scand J Immunol Suppl. 1973;1:47-56 PMID: 4797921
  13. Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure.
    Nucleic Acids Res. 1979 Nov 24;7(6):1541-52 PMID: 503860
  14. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  15. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  16. Disease-induced changes in the plasma binding of basic drugs.
    Clin Pharmacokinet. 1980 May-Jun;5(3):246-62 PMID: 6156048
  17. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen.
    Science. 1980 Jul 25;209(4455):497-9 PMID: 6248960
  18. Immunosuppression by human plasma alpha 1-acid glycoprotein: importance of the carbohydrate moiety.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):6109-13 PMID: 6934536
  19. The phenomenon of the acute phase response.
    Ann N Y Acad Sci. 1982;389:39-48 PMID: 7046585
  20. cAMP stimulates transcription of the gene for cytosolic phosphoenolpyruvate carboxykinase in rat liver nuclei.
    Proc Natl Acad Sci U S A. 1982 Sep;79(17):5137-41 PMID: 6291025
  21. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  22. Sequence of human haptoglobin cDNA: evidence that the alpha and beta subunits are coded by the same mRNA.
    Nucleic Acids Res. 1983 Sep 10;11(17):5811-9 PMID: 6310515
  23. Structure and in vitro transcription of a human H4 histone gene.
    Nucleic Acids Res. 1983 Oct 25;11(20):7069-86 PMID: 6314274
  24. Potential basis for regulation of the coordinately expressed fibrinogen genes: homology in the 5' flanking regions.
    Proc Natl Acad Sci U S A. 1984 Apr;81(8):2313-6 PMID: 6232608
  25. Human keratinocytes and monocytes release factors which regulate the synthesis of major acute phase plasma proteins in hepatic cells from man, rat, and mouse.
    J Biol Chem. 1984 Jun 10;259(11):7331-42 PMID: 6202694
  26. NF-kappa B: a pleiotropic mediator of inducible and tissue-specific gene control.
    Cell. 1989 Jul 28;58(2):227-9 PMID: 2665943
  27. Two distinct cellular phosphoproteins bind to the c-fos serum response element.
    EMBO J. 1989 Jun;8(6):1785-92 PMID: 2504586
  28. Interleukin 6 induces a liver-specific nuclear protein that binds to the promoter of acute-phase genes.
    Proc Natl Acad Sci U S A. 1989 Nov;86(21):8202-6 PMID: 2479021
  29. Alpha interferon and gamma interferon stimulate transcription of a single gene through different signal transduction pathways.
    Mol Cell Biol. 1989 Dec;9(12):5404-11 PMID: 2555698
  30. A multiple cytokine- and second messenger-responsive element in the enhancer of the human interleukin-6 gene: similarities with c-fos gene regulation.
    Mol Cell Biol. 1989 Dec;9(12):5537-47 PMID: 2511437
  31. Involvement of a NF-kappa B-like transcription factor in the activation of the interleukin-6 gene by inflammatory lymphokines.
    Mol Cell Biol. 1990 Feb;10(2):561-8 PMID: 2405250
  32. Constitutive and IL-6-induced nuclear factors that interact with the human C-reactive protein promoter.
    EMBO J. 1990 Feb;9(2):457-65 PMID: 2154372
  33. A 69-base-pair monkey DNA sequence enhances simian virus 40 replication and transcription through multiple motifs.
    J Virol. 1990 Mar;64(3):1360-5 PMID: 2154615
  34. An inducible 50-kilodalton NF kappa B-like protein and a constitutive protein both bind the acute-phase response element of the angiotensinogen gene.
    Mol Cell Biol. 1990 Mar;10(3):1023-32 PMID: 2106065
  35. Acute-phase reaction induces a specific complex between hepatic nuclear proteins and the interleukin 6 response element of the rat alpha 2-macroglobulin gene.
    Proc Natl Acad Sci U S A. 1990 Mar;87(6):2364-8 PMID: 1690431
  36. High level transient expression of a chloramphenicol acetyl transferase gene by DEAE-dextran mediated DNA transfection coupled with a dimethyl sulfoxide or glycerol shock treatment.
    Nucleic Acids Res. 1984 Jul 25;12(14):5707-17 PMID: 6589587
  37. Plasma protease inhibitors in mouse and man: divergence within the reactive centre regions.
    Nature. 1984 Sep 13-19;311(5982):175-7 PMID: 6547997
  38. Human triosephosphate isomerase cDNA and protein structure. Studies of triosephosphate isomerase deficiency in man.
    J Biol Chem. 1985 Mar 25;260(6):3748-53 PMID: 2579079
  39. Rat alpha 1-acid glycoprotein. Gene sequence and regulation by glucocorticoids in transfected L-cells.
    J Biol Chem. 1985 Apr 10;260(7):4397-403 PMID: 3838547
  40. Structure of the human alpha 1-acid glycoprotein gene: sequence homology with other human acute phase protein genes.
    Nucleic Acids Res. 1985 Jun 11;13(11):3941-52 PMID: 2409529
  41. Acute phase mediators and glucocorticoids elevate alpha 1-acid glycoprotein gene transcription.
    J Biol Chem. 1985 Dec 15;260(29):15386-9 PMID: 2415519
  42. Multiple sequence motifs are involved in SV40 enhancer function.
    EMBO J. 1986 Feb;5(2):387-97 PMID: 3011406
  43. Tissue-specific in vitro transcription from the mouse albumin promoter.
    Cell. 1986 Dec 5;47(5):767-76 PMID: 3779841
  44. Localization of DNA sequences involved in dexamethasone-dependent expression of the rat alpha 1-acid glycoprotein gene.
    Mol Cell Biol. 1986 Jul;6(7):2551-61 PMID: 3023939
  45. Sequence of rat liver alpha 2-macroglobulin and acute phase control of its messenger RNA.
    J Biol Chem. 1987 Jan 5;262(1):446-54 PMID: 2432068
  46. Distinct sets of acute phase plasma proteins are stimulated by separate human hepatocyte-stimulating factors and monokines in rat hepatoma cells.
    J Biol Chem. 1987 Jul 15;262(20):9756-68 PMID: 2439511
  47. Induction of fibrinogen and a subset of acute phase response genes involves a novel monokine which is mimicked by phorbol esters.
    J Biol Chem. 1987 Aug 5;262(22):10850-4 PMID: 2440878
  48. The human haptoglobin gene: transcriptional regulation during development and acute phase induction.
    EMBO J. 1987 Jul;6(7):1905-12 PMID: 2820712
  49. Structure of a human serum amyloid A gene and modulation of its expression in transfected L cells.
    J Biol Chem. 1987 Nov 15;262(32):15790-5 PMID: 2890635
  50. Identification and characterization of functional genes encoding the mouse major urinary proteins.
    Mol Cell Biol. 1987 Oct;7(10):3705-12 PMID: 2824995
  51. Interaction among hepatocyte-stimulating factors, interleukin 1, and glucocorticoids for regulation of acute phase plasma proteins in human hepatoma (HepG2) cells.
    J Immunol. 1987 Dec 15;139(12):4122-8 PMID: 2447159
  52. A high-efficiency HeLa cell nuclear transcription extract.
    DNA. 1988 Jan-Feb;7(1):47-55 PMID: 3349904
  53. Stimulation of murine hemopoietic colony formation by human IL-6.
    J Immunol. 1988 May 1;140(9):3040-4 PMID: 3258892
  54. Identification of sequences responsible for acute-phase induction of human C-reactive protein.
    Nucleic Acids Res. 1988 Apr 25;16(8):3195-207 PMID: 3375054
  55. Induction of rat acute-phase proteins by interleukin 6 in vivo.
    Eur J Immunol. 1988 May;18(5):717-21 PMID: 2454191
  56. Activation of transcription factor IIIC by the adenovirus E1A protein.
    Cell. 1988 Jun 17;53(6):907-20 PMID: 2968157
  57. Interleukin-1 stimulates diacylglycerol production in T lymphocytes by a novel mechanism.
    Cell. 1988 Jul 1;54(1):73-81 PMID: 2898301
  58. Complexity of the early genetic response to growth factors in mouse fibroblasts.
    Mol Cell Biol. 1988 May;8(5):2140-8 PMID: 2898731
  59. Phosphorylation-induced binding and transcriptional efficacy of nuclear factor CREB.
    Nature. 1988 Aug 11;334(6182):494-8 PMID: 2900470
  60. Yeast heat shock factor is an essential DNA-binding protein that exhibits temperature-dependent phosphorylation.
    Cell. 1988 Sep 9;54(6):855-64 PMID: 3044613
  61. Acute phase proteins as disease markers.
    Dis Markers. 1987 Mar;5(1):1-11 PMID: 2458880
  62. Phosphorylation of serum response factor, a factor that binds to the serum response element of the c-FOS enhancer.
    Proc Natl Acad Sci U S A. 1988 Oct;85(19):7206-10 PMID: 2845402
  63. Phorbol ester modulates interleukin 6- and interleukin 1-regulated expression of acute phase plasma proteins in hepatoma cells.
    J Biol Chem. 1988 Nov 25;263(33):17390-6 PMID: 2460462
  64. Gene regulation by steroid hormones.
    Cell. 1989 Feb 10;56(3):335-44 PMID: 2644044
  65. Cyclic AMP-dependent protein kinase phosphorylates and inactivates the yeast transcriptional activator ADR1.
    Cell. 1989 Feb 10;56(3):409-19 PMID: 2644045
  66. Hepatic acute phase reaction in vivo and in vitro.
    In Vitro Cell Dev Biol. 1989 Feb;25(2):115-26 PMID: 2466031
  67. Stimulation of hepatic acute phase response by cytokines and glucocorticoids.
    Ann N Y Acad Sci. 1989;557:280-95, discussion 295-6 PMID: 2472090
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.10.8.3965-3978.1990
Published
1990-08-00
Pages
3965-78
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC360907
Subset
IM
Grants
NCI NIH HHS · CA26122 · United States
External Links
PubMed source DOI: 10.1128/mcb.10.8.3965-3978.1990 PMC Full Text View journal details
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