Abstract
Recurrent chromosomal translocations involving the mixed lineage leukaemia (MLL) gene initiate aggressive forms of leukaemia, which are often refractory to conventional therapies. Many MLL-fusion partners are members of the super elongation complex (SEC), a critical regulator of transcriptional elongation, suggesting that aberrant control of this process has an important role in leukaemia induction. Here we use a global proteomic strategy to demonstrate that MLL fusions, as part of SEC and the polymerase-associated factor complex (PAFc), are associated with the BET family of acetyl-lysine recognizing, chromatin 'adaptor' proteins. These data provided the basis for therapeutic intervention in MLL-fusion leukaemia, via the displacement of the BET family of proteins from chromatin. We show that a novel small molecule inhibitor of the BET family, GSK1210151A (I-BET151), has profound efficacy against human and murine MLL-fusion leukaemic cell lines, through the induction of early cell cycle arrest and apoptosis. I-BET151 treatment in two human leukaemia cell lines with different MLL fusions alters the expression of a common set of genes whose function may account for these phenotypic changes. The mode of action of I-BET151 is, at least in part, due to the inhibition of transcription at key genes (BCL2, C-MYC and CDK6) through the displacement of BRD3/4, PAFc and SEC components from chromatin. In vivo studies indicate that I-BET151 has significant therapeutic value, providing survival benefit in two distinct mouse models of murine MLL-AF9 and human MLL-AF4 leukaemia. Finally, the efficacy of I-BET151 against human leukaemia stem cells is demonstrated, providing further evidence of its potent therapeutic potential. These findings establish the displacement of BET proteins from chromatin as a promising epigenetic therapy for these aggressive leukaemias.
MeSH Terms
Animals
Cell Line, Tumor
Chromatin/genetics,metabolism
Chromatin Immunoprecipitation
Disease Models, Animal
Gene Expression Profiling
Gene Expression Regulation, Neoplastic/drug effects
Heterocyclic Compounds, 4 or More Rings/pharmacology,therapeutic use
Humans
Leukemia, Myeloid, Acute/drug therapy,genetics,metabolism,pathology
Mice
Models, Molecular
Multiprotein Complexes/chemistry,metabolism
Myeloid-Lymphoid Leukemia Protein/metabolism
Oncogene Proteins, Fusion/metabolism
Protein Binding/drug effects
Proteomics
Transcription Factors/antagonists & inhibitors,metabolism
Transcription, Genetic/drug effects
Chemicals
Chromatin
GSK1210151A
Heterocyclic Compounds, 4 or More Rings
Multiprotein Complexes
Oncogene Proteins, Fusion
Transcription Factors
Myeloid-Lymphoid Leukemia Protein
Authors & Affiliations
27 authors, click to expand affiliations / ORCID
Dawson Mark A
Department of Haematology, Cambridge Institute for Medical Research and Addenbrookes Hospital, University of Cambridge, Cambridge CB2 0XY, UK.
Prinjha Rab K
Dittmann Antje
Giotopoulos George
Bantscheff Marcus
Chan Wai-In
Robson Samuel C
Chung Chun-wa
Hopf Carsten
Savitski Mikhail M
Huthmacher Carola
Gudgin Emma
Lugo Dave
Beinke Soren
Chapman Trevor D
Roberts Emma J
Soden Peter E
Auger Kurt R
Mirguet Olivier
Doehner Konstanze
Delwel Ruud
Burnett Alan K
Jeffrey Phillip
Drewes Gerard
Lee Kevin
Huntly Brian J P
Kouzarides Tony
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