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PMID: 21943198 Published · epublish English Journal Article Research Support, Non-U.S. Gov't

The HIV-1 gp120/V3 modifies the response of uninfected CD4 T cells to antigen presentation: mapping of the specific transcriptional signature.

Journal of translational medicine ·Vol. 9 ·2011-09-24 ·Pages 160

Morou AK, Porichis F, Krambovitis E, Sourvinos G, Spandidos DA, Zafiropoulos A

Abstract

The asymptomatic phase of HIV-1 infection is characterized by a progressive depletion of uninfected peripheral effector/memory CD4+ T cells that subsequently leads to immune dysfunction and AIDS symptoms. We have previously demonstrated that the presence of specific gp120/V3 peptides during antigen presentation can modify the activation of normal T-cells leading to altered immune function. The aim of the present study was to map the specific transcriptional profile invoked by an HIV-1/V3 epitope in uninfected T cells during antigen presentation. We exposed primary human peripheral blood monocytes to V3 lipopeptides using a liposome delivery system followed by a superantigen-mediated antigen presentation system. We then evaluated the changes in the T-cell transcriptional profile using oligonucleotide microarrays and performed Ingenuity Pathway Analysis (IPA) and DAVID analysis. The results were validated using realtime PCR, FACS, Western blotting and immunofluorescence. Our results revealed that the most highly modulated transcripts could almost entirely be categorized as related to the cell cycle or transcriptional regulation. The most statistically significant enriched categories and networks identified by IPA were associated with cell cycle, gene expression, immune response, infection mechanisms, cellular growth, proliferation and antigen presentation. Canonical pathways involved in energy and cell cycle regulation, and in the co-activation of T cells were also enriched. Taken together, these results document a distinct transcriptional profile invoked by the HIV-1/V3 epitope. These data could be invaluable to determine the underlying mechanism by which HIV-1 epitopes interfere with uninfected CD4+ T-cell function causing hyper proliferation and AICD.

MeSH Terms
Antigen Presentation/genetics,immunology CD4-Positive T-Lymphocytes/immunology Cell Cycle/genetics Cell Proliferation Cluster Analysis Epitopes/immunology Fluorescent Antibody Technique Gene Expression Profiling Gene Expression Regulation/immunology Gene Regulatory Networks/genetics HIV Envelope Protein gp120/immunology HIV Infections/genetics,immunology,pathology HIV-1/immunology Humans Ki-67 Antigen/metabolism Molecular Sequence Annotation Peptide Fragments/immunology Signal Transduction/genetics Transcription, Genetic
Chemicals
Epitopes HIV Envelope Protein gp120 HIV envelope protein gp120 (305-321) Ki-67 Antigen Peptide Fragments
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Morou Antigone K
Department of Virology, Medical School, University of Crete, Heraklion, Crete, Greece.
Porichis Filippos
Krambovitis Elias
Sourvinos George
Spandidos Demetrios A
Zafiropoulos Alexandros
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Article Info
Journal
Journal of translational medicine
Abbr.
J Transl Med
ISSN
1479-5876
DOI
10.1186/1479-5876-9-160
Published
2011-09-24
Epub
2011-00-24
Pages
160
Language
English
Region
England
NLM ID
101190741
PMCID
PMC3203262
Subset
IM
External Links
PubMed source DOI: 10.1186/1479-5876-9-160 PMC Full Text View journal details
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