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PMID: 21909100 Published · epublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Light-based feedback for controlling intracellular signaling dynamics.

Nature methods ·Vol. 8 ·No. 10 ·2011-09-11 ·Pages 837-9

Toettcher JE, Gong D, Lim WA, Weiner OD

Abstract

The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.

MeSH Terms
Animals Cell Membrane/drug effects,metabolism Computational Biology Feedback, Physiological/radiation effects Humans Light Models, Biological Phosphatidylinositol 3-Kinases/chemistry,metabolism Phosphoinositide-3 Kinase Inhibitors Protein Binding Signal Transduction/radiation effects
Chemicals
Phosphoinositide-3 Kinase Inhibitors
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Toettcher Jared E
Cardiovascular Research Institute and Department of Biochemistry, University of California San Francisco, San Francisco, California, USA.
Gong Delquin
Lim Wendell A
Weiner Orion D
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Article Info
Journal
Nature methods
Abbr.
Nat Methods
ISSN
1548-7105
DOI
10.1038/nmeth.1700
Published
2011-09-11
Epub
2011-00-11
Pages
837-9
Language
English
Region
United States
NLM ID
101215604
PMCID
PMC3184382
Subset
IM
Grants
NIGMS NIH HHS · R01 GM062583-08 · United States
NIGMS NIH HHS · P50 GM081879-01A2 · United States
NEI NIH HHS · PN2 EY016546-06 · United States
NIGMS NIH HHS · GM55040 · United States
NIGMS NIH HHS · R01 GM084040-04 · United States
NIGMS NIH HHS · R01 GM055040 · United States
NIGMS NIH HHS · R01 GM096164 · United States
NEI NIH HHS · PN2 EY016546 · United States
NIGMS NIH HHS · R01 GM055040-12 · United States
NEI NIH HHS · EY016546 · United States
NIGMS NIH HHS · R01 GM084040 · United States
NIGMS NIH HHS · P50GM081879 · United States
Howard Hughes Medical Institute · United States
NIGMS NIH HHS · P50 GM081879 · United States
NEI NIH HHS · PN1 EY016546 · United States
NIGMS NIH HHS · R01 GM062583 · United States
NIGMS NIH HHS · GM62583 · United States
NIGMS NIH HHS · GM084040 · United States
Corrections
CommentIn
PMID: 21959135
External Links
PubMed source DOI: 10.1038/nmeth.1700 PMC Full Text View journal details
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