Cloning and sequence analysis of tryptophan synthetase genes of an extreme thermophile, Thermus thermophilus HB27: plasmid transfer from replica-plated Escherichia coli recombinant colonies to competent T. thermophilus cells.

Journal of bacteriology ·Vol. 172 ·No. 6 ·1990-06-00 ·Pages 3490-5

Koyama Y, Furukawa K

Abstract

Tryptophan synthetase genes (trpBA) of the extreme thermophile Thermus thermophilus HB27 were cloned by a novel method of direct plasmid transfer from replica-plated Escherichia coli recombinant colonies to competent T. thermophilus HB27 trpB cells. The nucleotide sequences of the trpBA genes were determined. The amino acid sequences deduced from the nucleotide sequences of Thermus trpB and trpA were found to have identities of 54.8 and 28.7%, respectively, with those of E. coli trpB and trpA genes. Low cysteine content (one in trpB; zero in trpA) is a striking feature of these proteins, which may contribute to their thermostability.

MeSH Terms

Amino Acid Sequence Base Composition Base Sequence Cloning, Molecular Codon Escherichia coli/genetics Molecular Sequence Data Plasmids Recombination, Genetic Thermus/genetics Transfection Tryptophan Synthase/analysis,genetics

Chemicals

Codon Tryptophan Synthase

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Koyama Y

Fermentation Research Institute, AIST, MITI, Ibaraki, Japan.

Furukawa K

References (21)

21 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.172.6.3490-3495.1990

Published

1990-06-00

Pages

3490-5

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC209164

Subset

Databases

GENBANK

M32108

PIR

M32108

External Links

PubMed source DOI: 10.1128/jb.172.6.3490-3495.1990 PMC Full Text View journal details

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Cloning and sequence analysis of tryptophan synthetase genes of an extreme thermophile, Thermus thermophilus HB27: plasmid transfer from replica-plated Escherichia coli recombinant colonies to competent T. thermophilus cells.

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