Home LiteratureArticle Details
PMID: 2492495 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence of the Pseudomonas aeruginosa trpI activator gene and relatedness of trpI to other procaryotic regulatory genes.

Journal of bacteriology ·Vol. 171 ·No. 1 ·1989-01-00 ·Pages 172-83

Chang M, Hadero A, Crawford IP

Abstract

In Pseudomonas aeruginosa, the trpI gene product regulates the expression of the trpBA gene pair encoding tryptophan synthase. trpI and trpBA are transcribed divergently. The trpI DNA sequence and deduced amino acid sequence were determined. The trpI start codon was found to be 103 base pairs from that of trpB. trpI encodes a 293-residue protein and the size of the trpI gene product, measured on sodium dodecyl sulfatepolyacrylamide gels, was close to that calculated from the amino acid sequence. The amino acid sequence of trpI resembles that of Enterobacter cloacae ampR, the regulatory gene for the ampC cephalosporinase. The N-terminal portions of trpI and ampR resemble corresponding portions of ilvY, metR, and lysR in Escherichia coli and nodD in Rhizobium meliloti. This resemblance may help to define a trpI-related family of activator proteins sharing a common structural plan.

MeSH Terms
Amino Acid Sequence Base Sequence Escherichia coli/genetics Gene Expression Regulation Genes Genes, Bacterial Genes, Regulator Molecular Sequence Data Plasmids Pseudomonas aeruginosa/enzymology,genetics Restriction Mapping Sequence Homology, Nucleic Acid Tryptophan Synthase/biosynthesis,genetics
Chemicals
Tryptophan Synthase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Chang M
Department of Microbiology, University of Iowa, Iowa City 52242.
Hadero A
Crawford I P
References (38)
38 references, click to expand
  1. Ordering tryptophan synthase genes of Pseudomonas aeruginosa by cloning in Escherichia coli.
    J Bacteriol. 1981 Apr;146(1):102-7 PMID: 6260735
  2. Construction of biologically functional bacterial plasmids in vitro.
    Proc Natl Acad Sci U S A. 1973 Nov;70(11):3240-4 PMID: 4594039
  3. Partial amino acid sequence of penicillinase coded by Escherichia coli plasmid R6K.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3732-6 PMID: 358199
  4. Synthesis of tryptophan from chorismate: comparative aspects.
    Methods Enzymol. 1987;142:293-300 PMID: 3298977
  5. Host-specific regulation of nodulation genes in Rhizobium is mediated by a plant-signal, interacting with the nodD gene product.
    EMBO J. 1987 Apr;6(4):841-8 PMID: 16453758
  6. Systematic method for the detection of potential lambda Cro-like DNA-binding regions in proteins.
    J Mol Biol. 1987 Apr 5;194(3):557-64 PMID: 3625774
  7. Acetylornithinase of Escherichia coli: partial purification and some properties.
    J Biol Chem. 1956 Jan;218(1):97-106 PMID: 13278318
  8. Mechanism of araC autoregulation and the domains of two overlapping promoters, Pc and PBAD, in the L-arabinose regulatory region of Escherichia coli.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):752-6 PMID: 6262769
  9. Construction and characterization of the chloramphenicol-resistance gene cartridge: a new approach to the transcriptional mapping of extrachromosomal elements.
    Gene. 1982 Dec;20(2):305-16 PMID: 6299895
  10. A new and improved method for 3'-end labelling DNA using [alpha-32P]ddATP.
    Gene. 1984 Mar;27(3):309-13 PMID: 6735176
  11. A DNA sequence analysis package for the IBM personal computer.
    Nucleic Acids Res. 1984 Jan 11;12(1 Pt 2):605-14 PMID: 6546433
  12. Analysis of recombinant DNA using Escherichia coli minicells.
    Methods Enzymol. 1983;101:347-62 PMID: 6350817
  13. A large family of bacterial activator proteins.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6602-6 PMID: 3413113
  14. Regulation of diaminopimelate decarboxylase synthesis in Escherichia coli. III. Nucleotide sequence and regulation of the lysR gene.
    J Mol Biol. 1983 Aug 5;168(2):333-50 PMID: 6350602
  15. Rapid and sensitive protein similarity searches.
    Science. 1985 Mar 22;227(4693):1435-41 PMID: 2983426
  16. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  17. Structure and regulation of the anthranilate synthase genes in Pseudomonas aeruginosa: I. Sequence of trpG encoding the glutamine amidotransferase subunit.
    Mol Biol Evol. 1986 Sep;3(5):436-48 PMID: 3127654
  18. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
  19. MINIATURE escherichia coli CELLS DEFICIENT IN DNA.
    Proc Natl Acad Sci U S A. 1967 Feb;57(2):321-6 PMID: 16591472
  20. The regulation of tryptophan biosynthesis in Pseudomonas aeruginosa.
    Mol Gen Genet. 1973 Mar 1;121(2):117-32 PMID: 4632776
  21. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  22. Transcriptional activation at adjacent operators in the divergent-overlapping ilvY and ilvC promoters of Escherichia coli.
    J Mol Biol. 1988 Oct 5;203(3):643-63 PMID: 3062177
  23. Nucleotide sequence and in vivo expression of the ilvY and ilvC genes in Escherichia coli K12. Transcription from divergent overlapping promoters.
    J Biol Chem. 1986 Feb 15;261(5):2441-50 PMID: 3003115
  24. Nucleotide sequence of Rhizobium meliloti 1021 nodulation genes: nodD is read divergently from nodABC.
    DNA. 1985 Jun;4(3):241-8 PMID: 4006668
  25. Genetic evidence for a positive-acting regulatory factor mediating induction in the tryptophan pathway of Pseudomonas aeruginosa.
    J Mol Biol. 1982 Mar 25;156(1):67-77 PMID: 6808146
  26. Mutants of the catabolite activator protein of Escherichia coli that are specifically deficient in the gene-activation function.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8315-9 PMID: 2825186
  27. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  28. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid.
    J Bacteriol. 1978 Jun;134(3):1141-56 PMID: 149110
  29. Rhizobium japonicum USDA 191 has two nodD genes that differ in primary structure and function.
    J Bacteriol. 1988 Jan;170(1):12-20 PMID: 2826389
  30. Inducibility of tryptophan synthetase in Pseudomonas putida.
    Proc Natl Acad Sci U S A. 1966 Aug;56(2):717-24 PMID: 5229989
  31. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  32. Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3737-41 PMID: 358200
  33. Nucleotide sequence of the Salmonella typhimurium metR gene and the metR-metE control region.
    J Bacteriol. 1987 Sep;169(9):3932-7 PMID: 3040668
  34. Transcription of Rhizobium meliloti nodulation genes. Identification of a nodD transcription initiation site in vitro and in vivo.
    J Biol Chem. 1987 May 15;262(14):6849-55 PMID: 3032978
  35. Nucleotide sequence of the genes for tryptophan synthase in Pseudomonas aeruginosa.
    Mol Biol Evol. 1986 May;3(3):191-204 PMID: 3127651
  36. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  37. Wide ranging plasmid bearing the Pseudomonas aeruginosa tryptophan synthase genes.
    Nature. 1977 May 19;267(5608):283-4 PMID: 405620
  38. Homology between nucleotide sequences of promoter regions of nah and sal operons of NAH7 plasmid of Pseudomonas putida.
    Proc Natl Acad Sci U S A. 1986 Jan;83(2):369-73 PMID: 3001734
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.1.172-183.1989
Published
1989-01-00
Pages
172-83
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC209570
Subset
IM
Grants
NCRR NIH HHS · 1 U41 RRO1685 · United States
NIAID NIH HHS · AI20279 · United States
Databases
GENBANK
M21093
External Links
PubMed source DOI: 10.1128/jb.171.1.172-183.1989 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2