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PMID: 2186177 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Constitutive expression of human immunodeficiency virus (HIV) nef protein in human astrocytes does not influence basal or induced HIV long terminal repeat activity.

Journal of virology ·Vol. 64 ·No. 6 ·1990-06-00 ·Pages 3059-62

Bachelerie F, Alcami J, Hazan U, Israël N, Goud B, Arenzana-Seisdedos F, Virelizier JL

Abstract

Since human immunodeficiency virus (HIV) nef has been suggested to exert regulatory effects on HIV long terminal repeat (LTR) activity, we transiently transfected HIV LTR chloramphenicol acetyltransferase or luciferase expression vectors into a human astrocytoma clone (U-373nef) that constitutively expresses the HIV nef gene. In these cells, basal HIV LTR activity, as well as tumor necrosis factor-induced or tat-driven activity, was similar to that in control cells. Lack of any detectable effect of HIV nef on LTR activity was not the result of mutations in integrated nef DNA, as was shown by polymerase chain reaction. These data suggest that the role of nef in HIV genome transcription does not necessarily involve a direct influence on HIV LTR activity.

MeSH Terms
Astrocytoma Cell Line Gene Products, nef/genetics Genes, Viral Genes, nef HIV-1/genetics Humans Nucleic Acid Hybridization Plasmids Repetitive Sequences, Nucleic Acid Restriction Mapping Transfection Viral Regulatory and Accessory Proteins/genetics nef Gene Products, Human Immunodeficiency Virus
Chemicals
Gene Products, nef Viral Regulatory and Accessory Proteins nef Gene Products, Human Immunodeficiency Virus
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Bachelerie F
Laboratoire d'Immunologie Virale, Institut Pasteur, Paris, France.
Alcami J
Hazan U
Israël N
Goud B
Arenzana-Seisdedos F
Virelizier J L
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.6.3059-3062.1990
Published
1990-06-00
Pages
3059-62
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249492
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.6.3059-3062.1990 PMC Full Text View journal details
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