6 beta-Bromopenicillanic acid inactivates beta-lactamase I.

The Biochemical journal ·Vol. 177 ·No. 1 ·1979-01-01 ·Pages 365-7

Knott-Hunziker V, Orlek BS, Sammes PG, Waley SG

Abstract

The inactivation of beta-lactamase I by preparations of 6alpha-bromopenicillanic acid showed unexpected kinetic features that indicated that the active species was the 6beta-epimer. Samples containing 6beta-bromopenicillanic acid have been synthesized and shown to inactivate the enzyme in a rapid stoicheiometric reaction.

MeSH Terms

Chemical Phenomena Chemistry Kinetics Penicillanic Acid/pharmacology beta-Lactamase Inhibitors

Chemicals

beta-Lactamase Inhibitors Penicillanic Acid

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Knott-Hunziker V

Orlek B S

Sammes P G

Waley S G

References (10)

10 references, click to expand

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J Mol Biol. 1978 Apr 15;120(3):447-9 PMID: 418181
Substrate-induced deactivation of penicillinases. Studies of beta-lactamase I by hydrogen exchange.
Biochem J. 1977 Aug 1;165(2):279-85 PMID: 921750
A spectrophotometric assay of beta-lactamase action on penicillins.
Biochem J. 1974 Jun;139(3):789-90 PMID: 4368359
Separation, purification and properties of beta-lactamase I and beta-lactamase II from Bacillus cereus 569/H/9.
Biochem J. 1974 Oct;143(1):115-27 PMID: 4219278
The pH-dependence and group modification of beta-lactamase I.
Biochem J. 1975 Sep;149(3):547-51 PMID: 996
Synthesis of tritium-labelled isopenicillin N, penicillin N and 6-aminopenicillanic acid.
Biochem J. 1975 Dec;151(3):729-39 PMID: 175787
The partial amino acid sequence of the extracellular beta-lactamase I of Bacillus cereus 569/H.
Biochem J. 1975 May;147(2):313-26 PMID: 810136
Isomerisation of benzyl 6-phenoxyacetoxypenicillanates and their S-oxides.
J Chem Soc Perkin 1. 1976;(6):704-10 PMID: 943414
The amino acid sequence of Staphylococcus aureus penicillinase.
Biochem J. 1975 Nov;151(2):197-218 PMID: 1218078

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1770365

Published

1979-01-01

Pages

365-7

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1186377

Subset

External Links

PubMed source DOI: 10.1042/bj1770365 PMC Full Text View journal details

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6 beta-Bromopenicillanic acid inactivates beta-lactamase I.

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