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PMID: 21826462 Published · ppublish English Journal Article Review

Function of MbtH homologs in nonribosomal peptide biosynthesis and applications in secondary metabolite discovery.

Journal of industrial microbiology & biotechnology ·Vol. 38 ·No. 11 ·2011-11-00 ·Pages 1747-60

Baltz RH

Abstract

Mycobacterium tuberculosis encodes mycobactin, a peptide siderophore that is biosynthesized by a nonribosomal peptide synthetase (NRPS) mechanism. Within the mycobactin biosynthetic gene cluster is a gene that encodes a 71-amino-acid protein MbtH. Many other NRPS gene clusters harbor mbtH homologs, and recent genetic, biochemical, and structural studies have begun to shed light on the function(s) of these proteins. In some cases, MbtH-like proteins are required for biosynthesis of their cognate peptides, and non-cognate MbtH-like proteins have been shown to be partially complementary. Biochemical studies revealed that certain MbtH-like proteins participate in tight binding to NRPS proteins containing adenylation (A) domains where they stimulate adenylation reactions. Expression of MbtH-like proteins is important for a number of applications, including optimal production of native and genetically engineered secondary metabolites produced by mechanisms that employ NRPS enzymes. They also may serve as beacons to identify gifted actinomycetes and possibly other bacteria that encode multiple functional NRPS pathways for discovery of novel secondary metabolites by genome mining.

MeSH Terms
Actinobacteria/genetics,metabolism Amino Acid Sequence Bacteria/genetics,metabolism Bacterial Proteins/chemistry,genetics,physiology Fungal Proteins/genetics Molecular Sequence Data Mycobacterium tuberculosis/enzymology,genetics,metabolism Peptide Biosynthesis, Nucleic Acid-Independent Peptide Synthases/chemistry,genetics Siderophores/biosynthesis
Chemicals
Bacterial Proteins Fungal Proteins Siderophores Peptide Synthases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Baltz Richard H
CognoGen Biotechnology Consulting, 6438 North Olney Street, Indianapolis, IN 46220, USA. rbaltz923@aol.com
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Article Info
Journal
Journal of industrial microbiology & biotechnology
Abbr.
J Ind Microbiol Biotechnol
ISSN
1476-5535
DOI
10.1007/s10295-011-1022-8
Email
rbaltz923@aol.com
Published
2011-11-00
Epub
2011-00-09
Pages
1747-60
Language
English
Region
Germany
NLM ID
9705544
Subset
IM
External Links
PubMed source DOI: 10.1007/s10295-011-1022-8 View journal details
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