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PMID: 2180914 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequence analysis and expression of the two genes for elongation factor 1 alpha from the dimorphic yeast Candida albicans.

Journal of bacteriology ·Vol. 172 ·No. 4 ·1990-04-00 ·Pages 2036-45

Sundstrom P, Smith D, Sypherd PS

Abstract

Two Candida albicans genes that encode the protein synthesis factor elongation factor 1 alpha (EF-1 alpha) were cloned by using a heterologous TEF1 probe from Mucor racemosus to screen libraries of C. albicans genomic DNA. Sequence analysis of the two clones showed that regions of DNA flanking the coding regions of the two genes were not homologous, verifying the presence of two genes, called TEF1 and TEF2, for EF-1 alpha in C. albicans. The coding regions of TEF1 and TEF2 differed by only five nucleotides and encoded identical EF-1 alpha proteins of 458 amino acids. Both genes were transcribed into mRNA in vivo, as shown by hybridization of oligonucleotide probes, which bound specifically to the 3' nontranslated regions of TEF1 and TEF2, respectively, to C. albicans total RNA in Northern (RNA) blot analysis. The predicted EF-1 alpha protein of C. albicans was more similar to Saccharomyces cerevisiae EF-1 alpha than to M. racemosus EF-1 alpha. Furthermore, codon bias and the promoter and termination signals of the C. albicans EF-1 alpha proteins were remarkably similar to those of S. cerevisiae EF-1 alpha. Taken together, these results suggest that C. albicans is more closely related to the ascomycete S. cerevisiae than to the zygomycete M. racemosus.

MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern Candida albicans/genetics Cloning, Molecular Codon/genetics DNA, Fungal/genetics Escherichia coli/genetics Gene Expression Gene Library Genes, Fungal Molecular Sequence Data Peptide Elongation Factor 1 Peptide Elongation Factors/genetics RNA, Fungal/genetics Restriction Mapping Ribonucleoproteins/genetics Sequence Homology, Nucleic Acid
Chemicals
Codon DNA, Fungal Peptide Elongation Factor 1 Peptide Elongation Factors RNA, Fungal Ribonucleoproteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Sundstrom P
Department of Microbiology and Molecular Genetics, College of Medicine, University of California, Irvine 92717.
Smith D
Sypherd P S
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.4.2036-2045.1990
Published
1990-04-00
Pages
2036-45
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208702
Subset
IM
Grants
NIAID NIH HHS · AI25788 · United States
NIGMS NIH HHS · GM23999 · United States
Databases
GENBANK
M29934, M29935
External Links
PubMed source DOI: 10.1128/jb.172.4.2036-2045.1990 PMC Full Text View journal details
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