Home LiteratureArticle Details
PMID: 2175390 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

HSP12, a new small heat shock gene of Saccharomyces cerevisiae: analysis of structure, regulation and function.

Molecular & general genetics : MGG ·Vol. 223 ·No. 1 ·1990-08-00 ·Pages 97-106

Praekelt UM, Meacock PA

Abstract

We have isolated a new small heat shock gene, HSP12, from Saccharomyces cerevisiae. It encodes a polypeptide of predicted Mr 12 kDa, with structural similarity to other small heat shock proteins. HSP12 gene expression is induced several hundred-fold by heat shock and on entry into stationary phase. HSP12 mRNA is undetectable during exponential growth in rich medium, but low levels are present when cells are grown in minimal medium. Analysis of HSP12 expression in mutants affected in cAMP-dependent protein phosphorylation suggests that the gene is regulated by cAMP as well as heat shock. A disruption of the HSP12 coding region results in the loss of an abundant 14.4 kDa protein present in heat shocked and stationary phase cells. It also leads to the induction of the heat shock response under conditions normally associated with low-level HSP12 expression. The HSP12 disruption has no observable effect on growth at various temperatures, nor on the ability to acquire thermotolerance.

Related Genes
HSP12
MeSH Terms
Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Southern Cloning, Molecular Cyclic AMP/metabolism DNA, Fungal/genetics,isolation & purification Electrophoresis, Polyacrylamide Gel Exons Gene Expression Regulation, Fungal Genes, Fungal Heat-Shock Proteins/biosynthesis,genetics,metabolism Molecular Sequence Data Mutation Phosphorylation Saccharomyces cerevisiae/genetics,growth & development Sequence Homology, Nucleic Acid Temperature
Chemicals
DNA, Fungal Heat-Shock Proteins Cyclic AMP
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Praekelt U M
Leicester Biocentre, University of Leicester, England.
Meacock P A
References (50)
50 references, click to expand
  1. The yeast ubiquitin genes: a family of natural gene fusions.
    EMBO J. 1987 May;6(5):1429-39 PMID: 3038523
  2. Sequence and structural features associated with translational initiator regions in yeast--a review.
    Gene. 1987;59(1):1-18 PMID: 3325335
  3. Short repeated elements in the upstream regulatory region of the SUC2 gene of Saccharomyces cerevisiae.
    Mol Cell Biol. 1986 Jul;6(7):2324-33 PMID: 3097512
  4. Heat shock and the sorting of luminal ER proteins.
    EMBO J. 1989 Nov;8(11):3171-6 PMID: 2684638
  5. Lambda replacement vectors carrying polylinker sequences.
    J Mol Biol. 1983 Nov 15;170(4):827-42 PMID: 6315951
  6. Abnormal proteins serve as eukaryotic stress signals and trigger the activation of heat shock genes.
    Science. 1986 Apr 25;232(4749):522-4 PMID: 3083508
  7. Nucleotide sequence analysis of soybean small heat shock protein genes belonging to two different multigene families.
    J Mol Biol. 1988 Feb 20;199(4):549-57 PMID: 3351943
  8. Protein folding in mitochondria requires complex formation with hsp60 and ATP hydrolysis.
    Nature. 1989 Sep 14;341(6238):125-30 PMID: 2528694
  9. Mutations of the heat inducible 70 kilodalton genes of yeast confer temperature sensitive growth.
    Cell. 1984 Oct;38(3):841-9 PMID: 6386178
  10. Activation of the Drosophila hsp27 promoter by heat shock and by ecdysone involves independent and remote regulatory sequences.
    EMBO J. 1986 Jul;5(7):1653-8 PMID: 16453691
  11. DNA sequence required for efficient transcription termination in yeast.
    Cell. 1982 Mar;28(3):563-73 PMID: 6280875
  12. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  13. Purification and characterization of a heat-shock element binding protein from yeast.
    EMBO J. 1987 Oct;6(10):3035-41 PMID: 3319580
  14. The heat-shock proteins.
    Annu Rev Genet. 1988;22:631-77 PMID: 2853609
  15. Hsp26 is not required for growth at high temperatures, nor for thermotolerance, spore development, or germination.
    Cell. 1986 Jun 20;45(6):885-94 PMID: 3518952
  16. Yeast heat shock factor is an essential DNA-binding protein that exhibits temperature-dependent phosphorylation.
    Cell. 1988 Sep 9;54(6):855-64 PMID: 3044613
  17. Heat shock proteins and thermal resistance in yeast.
    Biochem Biophys Res Commun. 1980 Apr 14;93(3):819-24 PMID: 6992776
  18. Mistranslation induces the heat-shock response in the yeast Saccharomyces cerevisiae.
    Mol Microbiol. 1989 Feb;3(2):215-20 PMID: 2548059
  19. Genetic analysis of the role of cAMP in yeast.
    Yeast. 1985 Sep;1(1):15-24 PMID: 2851898
  20. Promoters, activator proteins, and the mechanism of transcriptional initiation in yeast.
    Cell. 1987 May 8;49(3):295-7 PMID: 2882858
  21. hsp82 is an essential protein that is required in higher concentrations for growth of cells at higher temperatures.
    Mol Cell Biol. 1989 Sep;9(9):3919-30 PMID: 2674684
  22. Codon usage in yeast: cluster analysis clearly differentiates highly and lowly expressed genes.
    Nucleic Acids Res. 1986 Jul 11;14(13):5125-43 PMID: 3526280
  23. A highly conserved sequence in yeast heat shock gene promoters.
    Nucleic Acids Res. 1988 Dec 23;16(24):11845 PMID: 3062587
  24. Heat shock proteins: the search for functions.
    J Cell Biol. 1986 Aug;103(2):321-5 PMID: 2426277
  25. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  26. Conservation of high efficiency promoter sequences in Saccharomyces cerevisiae.
    Nucleic Acids Res. 1982 Apr 24;10(8):2625-37 PMID: 6281737
  27. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  28. Yeast Hsp70 RNA levels vary in response to the physiological status of the cell.
    J Bacteriol. 1989 May;171(5):2680-8 PMID: 2651414
  29. S. cerevisiae encodes an essential protein homologous in sequence and function to mammalian BiP.
    Cell. 1989 Jun 30;57(7):1223-36 PMID: 2661019
  30. Small heat shock proteins in Drosophila may confer thermal tolerance.
    Exp Cell Res. 1983 Sep;147(2):437-42 PMID: 6193982
  31. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  32. The heat shock response is self-regulated at both the transcriptional and posttranscriptional levels.
    Cell. 1982 Dec;31(3 Pt 2):593-603 PMID: 7159929
  33. Delimitation and properties of DNA sequences required for the regulated expression of human interferon-beta gene.
    Cell. 1985 Jun;41(2):489-96 PMID: 2985279
  34. One-step gene disruption in yeast.
    Methods Enzymol. 1983;101:202-11 PMID: 6310324
  35. Complex interactions among members of an essential subfamily of hsp70 genes in Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Jul;7(7):2568-77 PMID: 3302682
  36. Dual regulation of the expression of the polyubiquitin gene by cyclic AMP and heat shock in yeast.
    EMBO J. 1988 Feb;7(2):495-502 PMID: 2835229
  37. Codon selection in yeast.
    J Biol Chem. 1982 Mar 25;257(6):3026-31 PMID: 7037777
  38. The heat-shock response.
    Annu Rev Biochem. 1986;55:1151-91 PMID: 2427013
  39. Human HSP70 promoter contains at least two distinct regulatory domains.
    Proc Natl Acad Sci U S A. 1986 Feb;83(3):629-33 PMID: 3456160
  40. A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides.
    Nature. 1988 Apr 28;332(6167):800-5 PMID: 3282178
  41. The yeast polyubiquitin gene is essential for resistance to high temperatures, starvation, and other stresses.
    Cell. 1987 Mar 27;48(6):1035-46 PMID: 3030556
  42. 70K heat shock related proteins stimulate protein translocation into microsomes.
    Nature. 1988 Apr 28;332(6167):805-10 PMID: 3282179
  43. Mutations in cognate genes of Saccharomyces cerevisiae hsp70 result in reduced growth rates at low temperatures.
    Mol Cell Biol. 1985 Dec;5(12):3517-24 PMID: 3915778
  44. Structure and expression of a yeast gene encoding the small heat-shock protein Hsp26.
    Gene. 1989 May 30;78(2):323-30 PMID: 2673926
  45. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.
    Gene. 1985;33(1):103-19 PMID: 2985470
  46. Chromatin-associated heat shock proteins of Dictyostelium.
    Dev Biol. 1982 Apr;90(2):412-8 PMID: 7075869
  47. Durable synthesis of high molecular weight heat shock proteins in G0 cells of the yeast and other eucaryotes.
    J Cell Biol. 1984 Jul;99(1 Pt 1):199-207 PMID: 6429154
  48. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  49. Four small Drosophila heat shock proteins are related to each other and to mammalian alpha-crystallin.
    Proc Natl Acad Sci U S A. 1982 Apr;79(7):2360-4 PMID: 6285380
  50. Sterile host yeasts (SHY): a eukaryotic system of biological containment for recombinant DNA experiments.
    Gene. 1979 Dec;8(1):17-24 PMID: 395030
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00315801
Published
1990-08-00
Pages
97-106
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00315801
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2