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PMID: 217363 Published · ppublish English Journal Article

Effects of cholera toxin and guanosine 5'-[betagamma-imido]triphosphate on beta-adrenergic-receptor affinity.

The Biochemical journal ·Vol. 176 ·No. 2 ·1978-11-15 ·Pages 505-10

Fischer J, Sharp GW

Abstract

A comparison was made of the effects of cholera toxin and p[NH]ppG on the binding affinity of beta-adrenergic receptors in toad erythrocyte membranes. This was determined by studying the ability of isoproterenol and propranolol to compete for the receptor with (-)-[3H]dihydroalprenolol. p[NH]ppG decreased the receptor affinity for the agonist isoproterenol (i.e. a 'right' shift in the displacement-concentration curve), but was without effect on the affinity for the antagonist propranolol. Toad erythrocyte membranes after treatment with cholera toxin exhibited increased receptor affinity for isoproterenol (i.e. a 'left' shift in the displacement curve), but did not affect the affinity for propranolol. p[NH[ppG was able to exert its right shift even in cholera-toxin treated membranes. The ability of cholera toxin to alter beta-adrenergic-receptor affinity is interpreted as further evidence that the toxin affects the nucleotide-regulatory component of adenylate cyclase. The regulatory component affected may be the catecholamine-sensitive guanosine triphosphatase.

MeSH Terms
Alprenolol/metabolism Animals Anura Cholera Toxin/pharmacology Erythrocyte Membrane/metabolism Erythrocytes/metabolism Guanosine Triphosphate/analogs & derivatives Guanylyl Imidodiphosphate/pharmacology Isoproterenol/metabolism Propranolol/metabolism Receptors, Adrenergic/drug effects Receptors, Adrenergic, beta/drug effects,metabolism
Chemicals
Receptors, Adrenergic Receptors, Adrenergic, beta Guanylyl Imidodiphosphate Guanosine Triphosphate Alprenolol Cholera Toxin Propranolol Isoproterenol
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Fischer J
Sharp G W
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26 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1760505
Published
1978-11-15
Pages
505-10
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1186259
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1760505 PMC Full Text View journal details
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