Cholera toxin requires oxidized nicotinamide-adenine dinucleotide to activate adenylate cyclase in purified rat liver plasma membranes.

The Biochemical journal ·Vol. 161 ·No. 3 ·1977-03-01 ·Pages 639-42

Martin BR, Houslay MD, Kennedy EL

Abstract

Activation of adenylate cyclase in isolated rat liver plasma membranes by cholera toxin was demonstrated. The activation requires the presence of NAD+ and ATP and is irreversible.

MeSH Terms

Adenosine Triphosphate/metabolism Adenylyl Cyclases/metabolism Animals Bacterial Toxins/pharmacology Cell Membrane/enzymology Enzyme Activation Liver/enzymology NAD/metabolism Rats Vibrio cholerae

Chemicals

Bacterial Toxins NAD Adenosine Triphosphate Adenylyl Cyclases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Martin B R

Houslay M D

Kennedy E L

References (10)

10 references, click to expand

Mechanism of activation of adenylate cyclase by cholera toxin.
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3438-42 PMID: 1059129
Involvement of nicotinamide adenine dinucleotide in the action of cholera toxin in vitro.
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2064-8 PMID: 166378
The mechanism of action of cholera toxin in pigeon erythrocyte lysates.
J Biol Chem. 1975 Aug 25;250(16):6424-32 PMID: 169243
Mechanism of activation of adenylate cyclase by Vibrio cholerae enterotoxin. Relations to the mode of activation by hormones.
J Membr Biol. 1975 Nov 7;24(2):107-29 PMID: 172636
The glucagon receptor of rat liver plasma membrane can couple to adenylate cyclase without activating it.
Biochim Biophys Acta. 1976 Jun 17;436(2):489-94 PMID: 179598
Role of adenine and guanine nucleotides in the activity and response of adenylate cyclase systems to hormones: evidence for multisite transition states.
Adv Cyclic Nucleotide Res. 1975;5:3-29 PMID: 236641
Effects of glucagon on cyclic AMP and carbohydrate metabolism in livers from diabetic rats.
Biochim Biophys Acta. 1974 Mar 20;343(1):250-67 PMID: 4364131
A highly sensitive adenylate cyclase assay.
Anal Biochem. 1974 Apr;58(2):541-8 PMID: 4827395
Mechanism of action of Vibrio cholerae enterotoxin. Effects on adenylate cyclase of toad and rat erythrocyte plasma membranes.
J Membr Biol. 1975 Jun 3;22(1):1-28 PMID: 805247
Mechanism of activation of adenylate cyclase by Vibrio cholerae enterotoxin.
J Membr Biol. 1975 Jun 3;22(1):29-52 PMID: 805248

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1610639

Published

1977-03-01

Pages

639-42

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1164551

Subset

External Links

PubMed source DOI: 10.1042/bj1610639 PMC Full Text View journal details

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Cholera toxin requires oxidized nicotinamide-adenine dinucleotide to activate adenylate cyclase in purified rat liver plasma membranes.

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