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PMID: 21684671 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Human U2B″ protein binding to snRNA stemloops.

Biophysical chemistry ·Vol. 159 ·No. 1 ·2011-11-00 ·Pages 82-9

Williams SG, Hall KB

Abstract

The human U2B″ protein is one of the unique proteins that comprise the U2 snRNP, but it is also a representative of the U1A/U2B″ protein family. In the U2 snRNP, it is bound to Stem-Loop IV (SLIV) of the U2 snRNA. We find that in vitro it binds not only to human SLIV, but also to Stem-Loop II (SLII) from human U1 snRNA and to Drosophila U2 snRNA SLIV. The thermodynamics of these binding interactions show a striking similarity, leading to the conclusion that U2B″ has a relaxed specificity for its RNA targets. The binding properties of U2B″ are distinct from those of human U1A and of Drosophila SNF, despite its high homology to those proteins, and so provide important new information on how this protein family has modulated its target preferences.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Drosophila Humans Models, Molecular Molecular Sequence Data Nucleic Acid Conformation Protein Binding RNA, Small Nuclear/chemistry,metabolism Thermodynamics snRNP Core Proteins/metabolism
Chemicals
RNA, Small Nuclear SNRPB2 protein, human U1 small nuclear RNA U2 small nuclear RNA snRNP Core Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Williams Sandra G
Department of Biochemistry and Molecular Biophysics, Washington University Medical School, St Louis, MO 63110, United States.
Hall Kathleen B
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Article Info
Journal
Biophysical chemistry
Abbr.
Biophys Chem
ISSN
1873-4200
DOI
10.1016/j.bpc.2011.05.010
Published
2011-11-00
Epub
2011-00-16
Pages
82-9
Language
English
Region
Netherlands
NLM ID
0403171
PMCID
PMC3166965
Subset
IM
Grants
NIGMS NIH HHS · F31 GM089576 · United States
NIGMS NIH HHS · F31 GM089576-02 · United States
NIGMS NIH HHS · R01 GM096444 · United States
External Links
PubMed source DOI: 10.1016/j.bpc.2011.05.010 PMC Full Text View journal details
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