Home LiteratureArticle Details
PMID: 2167660 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The liver cell plasma membrane Ca2+ inflow systems exhibit a broad specificity for divalent metal ions.

The Biochemical journal ·Vol. 269 ·No. 3 ·1990-08-01 ·Pages 579-87

Crofts JN, Barritt GJ

Abstract

1. The inflow of Mn2+ across the plasma membranes of isolated hepatocytes was monitored by measuring the quenching of the fluorescence of intracellular quin2, by atomic absorption spectroscopy and by the uptake of 54Mn2+. The inflow of other divalent metal ions was measured using quin2. 2. Under ionic conditions which resembled those present in the cytoplasmic space, Mn2+, Zn2+, Co2+, Ni2+ and Cd2+ each quenched the fluorescence of a solution of Ca2(+)-quin2. 3. The addition of Mn2+, Zn2+, Co2+, Ni2+ or Cd2+ to cells loaded with quin2 caused a time-dependent decrease in the fluorescence of intracellular quin2. Plots of the rate of decrease in fluorescence as a function of the concentration of Mn2+ reached a plateau at 100 microM-Mn2+. 4. The rate of decrease in fluorescence induced by Mn2+ was stimulated by 20% in the presence of vasopressin. The effect of vasopressin was completely inhibited by 200 microM-verapamil. Adrenaline, angiotensin II and glucagon also stimulated the rate of decrease in the fluorescence of intracellular quin2 induced by Mn2+. 5. The rate of decrease in fluorescence induced by Zn2+, Co2+, Ni2+ or Cd2+ was stimulated by between 20 and 190% in the presence of vasopressin or angiotensin II. 6. The rates of uptake of Mn2+ measured by atomic absorption spectroscopy or by using 54Mn2+ were inhibited by about 20% by 1.3 mM-Ca2+o and stimulated by 30% by vasopressin. 7. Plots of Mn2+ uptake, measured by atomic absorption spectroscopy or with 54Mn2+, as a function of the extracellular concentration of Mn2+ were biphasic over the range 0.05-1.0 mM added Mn2+ and did not reach a plateau at 1.0 mM-Mn2+. 8. It is concluded that (i) hepatocytes possess both a basal and a receptor-activated divalent cation inflow system, each of which has a broad specificity for metal ions, and (ii) the receptor-activated divalent cation inflow system is the receptor-operated Ca2+ channel.

MeSH Terms
Aminoquinolines/metabolism Animals Cadmium/pharmacokinetics,pharmacology Calcium/metabolism Calcium Channels/metabolism Cations, Divalent/pharmacokinetics Cell Membrane/metabolism,ultrastructure Cobalt/pharmacokinetics,pharmacology Dose-Response Relationship, Drug Fluorescence Liver/cytology,metabolism,ultrastructure Male Manganese/pharmacokinetics,pharmacology Metals/pharmacokinetics Rats Rats, Inbred Strains Spectrophotometry, Atomic Time Factors Zinc/pharmacokinetics,pharmacology
Chemicals
Aminoquinolines Calcium Channels Cations, Divalent Metals Cadmium Cobalt Manganese Zinc Quin2 Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Crofts J N
Department of Medical Biochemistry, Flinders University School of Medicine, Flinders Medical Centre, Bedford Park, Australia.
Barritt G J
References (53)
53 references, click to expand
  1. The stimulation by sodium fluoride of plasma-membrane Ca2+ inflow in isolated hepatocytes. Evidence that a GTP-binding regulatory protein is involved in the hormonal stimulation of Ca2+ inflow.
    Biochem J. 1987 Jul 1;245(1):41-7 PMID: 3117043
  2. The measurement of Ca2+ inflow across the liver cell plasma membrane by using quin2 and studies of the roles of Na+ and extracellular Ca2+ in the mechanism of Ca2+ inflow.
    Biochem J. 1989 Nov 15;264(1):61-70 PMID: 2604718
  3. Fluorescence and bioluminescence measurement of cytoplasmic free calcium.
    Biochem J. 1987 Dec 1;248(2):313-28 PMID: 3325037
  4. Evidence that a pertussis-toxin-sensitive substrate is involved in the stimulation by epidermal growth factor and vasopressin of plasma-membrane Ca2+ inflow in hepatocytes.
    Biochem J. 1987 Dec 15;248(3):911-8 PMID: 3501716
  5. Platelets and parotid acinar cells have different mechanisms for agonist-stimulated divalent cation entry.
    J Biol Chem. 1988 May 5;263(13):6161-4 PMID: 3360779
  6. Evidence that agonists stimulate bivalent-cation influx into human endothelial cells.
    Biochem J. 1988 Oct 1;255(1):179-84 PMID: 3196311
  7. Use of manganese to discriminate between calcium influx and mobilization from internal stores in stimulated human neutrophils.
    J Biol Chem. 1989 Jan 25;264(3):1522-7 PMID: 2536366
  8. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study.
    J Cell Biol. 1969 Dec;43(3):506-20 PMID: 4900611
  9. The initial velocities of calcium uptake by rat liver mitochondria.
    J Biol Chem. 1973 Aug 10;248(15):5527-31 PMID: 4768910
  10. Permeation of manganese, cadmium, zinc, and beryllium through calcium channels of an insect muscle membrane.
    Science. 1977 Apr 15;196(4287):309-11 PMID: 847472
  11. Properties of the calcium-extruding mechanism of liver cells.
    J Physiol. 1978 Aug;281:29-43 PMID: 359780
  12. Zinc-dependent action potentials in giant neurons of the snail, Euhadra quaestia.
    J Membr Biol. 1979 Sep 14;49(4):325-44 PMID: 480340
  13. Calcium channel.
    Annu Rev Neurosci. 1981;4:69-125 PMID: 6261668
  14. Membrane biophysics of calcium currents.
    Fed Proc. 1981 Jun;40(8):2220-5 PMID: 7238906
  15. A kinetic analysis of the effects of adrenaline on calcium distribution in isolated rat liver parenchymal cells.
    J Physiol. 1981 Mar;312:29-55 PMID: 7264996
  16. Evidence that lanthanum ions stimulate calcium inflow to isolated hepatocytes.
    Biochem J. 1981 Oct 15;200(1):109-14 PMID: 7332533
  17. Calcium homeostasis in intact lymphocytes: cytoplasmic free calcium monitored with a new, intracellularly trapped fluorescent indicator.
    J Cell Biol. 1982 Aug;94(2):325-34 PMID: 6980885
  18. Stimulation of phosphatidylinositol 4,5-bisphosphate hydrolysis in hepatocytes by vasopressin.
    J Biol Chem. 1983 Mar 10;258(5):2770-3 PMID: 6298203
  19. Free cytoplasmic calcium concentration and the mitogenic stimulation of lymphocytes.
    J Biol Chem. 1983 Apr 25;258(8):4876-82 PMID: 6601105
  20. A kinetic investigation of the effects of adrenaline on 45Ca2+ exchange in isolated hepatocytes at different Ca2+ concentrations, at 20 degrees C and in the presence of inhibitors of mitochondrial Ca2+ transport.
    Biochem J. 1983 Oct 15;216(1):51-62 PMID: 6651779
  21. Calcium pools in saponin-permeabilized guinea pig hepatocytes.
    J Biol Chem. 1983 Dec 25;258(24):15336-45 PMID: 6654915
  22. Cytosolic free Ca2+ in isolated rat hepatocytes as measured by quin2. Effects of noradrenaline and vasopressin.
    FEBS Lett. 1984 Feb 13;167(1):19-24 PMID: 6698202
  23. myo-Inositol 1,4,5-trisphosphate. A second messenger for the hormonal mobilization of intracellular Ca2+ in liver.
    J Biol Chem. 1984 Mar 10;259(5):3077-81 PMID: 6607924
  24. Relationship between inositol polyphosphate production and the increase of cytosolic free Ca2+ induced by vasopressin in isolated hepatocytes.
    J Biol Chem. 1984 May 10;259(9):5574-84 PMID: 6325442
  25. Agonists stimulate divalent cation channels in the plasma membrane of human platelets.
    FEBS Lett. 1985 Jul 8;186(2):175-9 PMID: 2408921
  26. Synergistic stimulation of the Ca2+ influx in rat hepatocytes by glucagon and the Ca2+-linked hormones vasopressin and angiotensin II.
    J Biol Chem. 1985 Sep 25;260(21):11635-42 PMID: 2995343
  27. The role of extracellular Ca2+ in the response of the hepatocyte to Ca2+-dependent hormones.
    J Biol Chem. 1985 Oct 15;260(23):12508-15 PMID: 4044600
  28. A model for receptor-regulated calcium entry.
    Cell Calcium. 1986 Feb;7(1):1-12 PMID: 2420465
  29. Pharmacology of calcium channels and smooth muscle.
    Annu Rev Pharmacol Toxicol. 1986;26:225-58 PMID: 2424362
  30. Studies with verapamil and nifedipine provide evidence for the presence in the liver cell plasma membrane of two types of Ca2+ inflow transporter which are dissimilar to potential-operated Ca2+ channels.
    Biochem Pharmacol. 1986 Sep 15;35(18):3045-52 PMID: 2428376
  31. Effects of vasopressin and La3+ on plasma-membrane Ca2+ inflow and Ca2+ disposition in isolated hepatocytes. Evidence that vasopressin inhibits Ca2+ disposition.
    Biochem J. 1986 Sep 15;238(3):793-800 PMID: 3099775
  32. Effect of extracellular Ca2+ on plasma membrane Ca2+ inflow and cytoplasmic free Ca2+ in isolated hepatocytes.
    Biochim Biophys Acta. 1987 Apr 22;928(2):208-16 PMID: 3105594
  33. The influx of Ca2+ induced by the administration of glucagon and Ca2+-mobilizing agents to the perfused rat liver could involve at least two separate pathways.
    Biochem J. 1987 Feb 15;242(1):43-50 PMID: 3496082
  34. The second messenger linking receptor activation to internal Ca release in liver.
    Nature. 1984 May 3-9;309(5963):63-6 PMID: 6325926
  35. Mechanism of ion permeation through calcium channels.
    Nature. 1984 May 31-Jun 6;309(5967):453-6 PMID: 6328315
  36. The action of alpha-adrenergic agonists on plasma-membrane calcium fluxes in perfused rat liver.
    Biochem J. 1984 May 15;220(1):43-50 PMID: 6743272
  37. Noradrenaline, vasopressin and angiotensin increase Ca2+ influx by opening a common pool of Ca2+ channels in isolated rat liver cells.
    Biochem J. 1984 Jul 1;221(1):121-7 PMID: 6087800
  38. Cytosolic Ca2+ homeostasis in Ehrlich and Yoshida carcinomas. A new, membrane-permeant chelator of heavy metals reveals that these ascites tumor cell lines have normal cytosolic free Ca2+.
    J Biol Chem. 1985 Mar 10;260(5):2719-27 PMID: 3919006
  39. Effect of depolarizing concentrations of potassium on calcium uptake and metabolism in rat liver.
    FEBS Lett. 1985 Apr 8;183(1):70-4 PMID: 3979569
  40. Assessment of effects of vasopressin, angiotensin II, and glucagon on Ca2+ fluxes and phosphorylase activity in liver.
    Methods Enzymol. 1985;109:550-8 PMID: 3990571
  41. Stimulation of inositol trisphosphate formation in hepatocytes by vasopressin, adrenaline and angiotensin II and its relationship to changes in cytosolic free Ca2+.
    Biochem J. 1985 Apr 1;227(1):79-90 PMID: 3873238
  42. Evidence that guanosine 5'-[gamma-thio]triphosphate stimulates plasma membrane Ca2+ inflow when introduced into hepatocytes.
    Biochem J. 1989 Jan 15;257(2):591-8 PMID: 2649079
  43. Capacitative calcium entry in parotid acinar cells.
    Biochem J. 1989 Mar 1;258(2):409-12 PMID: 2650680
  44. Influx of bivalent cations can be independent of receptor stimulation in human endothelial cells.
    Biochem J. 1989 Apr 1;259(1):125-9 PMID: 2541679
  45. How do inositol phosphates regulate calcium signaling?
    FASEB J. 1989 Jun;3(8):1899-905 PMID: 2542110
  46. Receptor-mediated calcium entry in fura-2-loaded human platelets stimulated with ADP and thrombin. Dual-wavelengths studies with Mn2+.
    Biochem J. 1989 Mar 15;258(3):923-6 PMID: 2730577
  47. Electrophysiological properties of isolated rat liver cells.
    J Cell Physiol. 1989 Jun;139(3):580-5 PMID: 2544611
  48. Voltage-gated calcium channels: direct observation of the anomalous mole fraction effect at the single-channel level.
    Proc Natl Acad Sci U S A. 1989 Jul;86(13):5207-11 PMID: 2544893
  49. Activation of calcium entry by the tumor promoter thapsigargin in parotid acinar cells. Evidence that an intracellular calcium pool and not an inositol phosphate regulates calcium fluxes at the plasma membrane.
    J Biol Chem. 1989 Jul 25;264(21):12266-71 PMID: 2663854
  50. Monitoring of the activation of receptor-operated calcium channels in human platelets.
    Biochem Biophys Res Commun. 1989 Jul 14;162(1):24-9 PMID: 2546547
  51. Vasopressin-stimulated Ca2+ influx in rat hepatocytes is inhibited in high-K+ medium.
    Biochem J. 1989 Jun 15;260(3):821-7 PMID: 2548488
  52. Inhibition of the liver cell receptor-activated Ca2+ inflow system by metal ion inhibitors of voltage-operated Ca2+ channels but not by other inhibitors of Ca2+ inflow.
    Biochim Biophys Acta. 1989 Oct 9;1013(3):197-205 PMID: 2553103
  53. Cadmium uptake and toxicity via voltage-sensitive calcium channels.
    J Biol Chem. 1987 Dec 5;262(34):16333-7 PMID: 2445745
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2690579
Published
1990-08-01
Pages
579-87
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1131626
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2690579 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2