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PMID: 6698202 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cytosolic free Ca2+ in isolated rat hepatocytes as measured by quin2. Effects of noradrenaline and vasopressin.

FEBS letters ·Vol. 167 ·No. 1 ·1984-02-13 ·Pages 19-24

Berthon B, Binet A, Mauger JP, Claret M

Abstract

Cytosolic [Ca2+] has been measured by using the Ca2+-sensitive indicator quin2 in rat liver cells. Optimal loading and hydrolysis have been obtained by equilibrating the cells with 50 microM quin2 acetoxymethyl ester for 150 s. The increase in [Ca2+]i initiated by noradrenaline and vasopressin was reduced but not abolished by removing external Ca2+.

MeSH Terms

Aminoquinolines Animals Calcium/analysis Fluorescent Dyes Liver/analysis,drug effects Norepinephrine/pharmacology Propranolol/pharmacology Rats Vasopressins/pharmacology

Chemicals

Aminoquinolines Fluorescent Dyes Vasopressins Quin2-acetoxymethyl ester Propranolol Calcium Norepinephrine

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Berthon B

Binet A

Mauger J P

Claret M

Article Info

Journal

FEBS letters

Abbr.

FEBS Lett

ISSN

0014-5793

DOI

10.1016/0014-5793(84)80824-8

Published

1984-02-13

Pages

19-24

Language

English

Region

England

NLM ID

0155157

Subset

External Links

PubMed source DOI: 10.1016/0014-5793(84)80824-8 View journal details

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Cytosolic free Ca2+ in isolated rat hepatocytes as measured by quin2. Effects of noradrenaline and vasopressin.

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