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PMID: 2160432 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Phenotypic evaluation of acapsular transposon mutants of Vibrio vulnificus.

Infection and immunity ·Vol. 58 ·No. 6 ·1990-06-00 ·Pages 1769-73

Wright AC, Simpson LM, Oliver JD, Morris JG

Abstract

Translucent, avirulent spontaneous phase variants of Vibrio vulnificus MO6-24 reverted back to the original opaque, encapsulated phenotype under both in vivo and in vitro conditions. Two translucent, acapsular mutants, which did not show phase variation, were constructed by using the transposon Tn5 IS50L::phoA (TnphoA). Loss of capsule was accompanied by decreases in virulence, hydrophilicity, and serum resistance. The ability to utilize transferrin-bound iron for growth was lost in only one of the two unencapsulated mutants. Our data emphasize the apparent importance of capsule in the virulence of V. vulnificus and indicate that utilization of transferrin-bound iron is independent of encapsulation.

MeSH Terms
Animals DNA Transposable Elements Mice Microscopy, Electron Mutation Phenotype Vibrio/genetics,pathogenicity,ultrastructure Vibrio Infections/microbiology
Chemicals
DNA Transposable Elements
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Wright A C
Department of Medicine, University of Maryland School of Medicine, Baltimore.
Simpson L M
Oliver J D
Morris J G
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.6.1769-1773.1990
Published
1990-06-00
Pages
1769-73
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258721
Subset
IM
Grants
NIAID NIH HHS · 1RO1AI22189-01A1 · United States
External Links
PubMed source DOI: 10.1128/iai.58.6.1769-1773.1990 PMC Full Text View journal details
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