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PMID: 21571221 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Regulated offloading of cytoplasmic dynein from microtubule plus ends to the cortex.

Developmental cell ·Vol. 20 ·No. 5 ·2011-05-17 ·Pages 639-51

Markus SM, Lee WL

Abstract

Cytoplasmic dynein mediates spindle orientation from the cell cortex through interactions with astral microtubules, but neither the mechanism governing its cortical targeting nor the regulation thereof is well understood. Here we show that yeast dynein offloads from microtubule plus ends to the daughter cell cortex. Mutants with an engineered peptide inserted between the tail domain and the motor head retain wild-type motor activity but exhibit enhanced offloading and cortical targeting. Conversely, shortening the "neck" sequence between the tail and motor domains precludes offloading from the microtubule plus ends. Furthermore, chimeric mutants with mammalian dynein "neck" sequences rescue targeting and function. These findings provide direct support for an active microtubule-mediated delivery process that appears to be regulated by a conserved masking/unmasking mechanism.

MeSH Terms
Cytoplasmic Dyneins/metabolism Cytoskeletal Proteins/metabolism Microtubules/metabolism Mutation Saccharomyces cerevisiae/cytology,metabolism Saccharomyces cerevisiae Proteins/metabolism
Chemicals
Cytoskeletal Proteins NUM1 protein, S cerevisiae Saccharomyces cerevisiae Proteins Cytoplasmic Dyneins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Markus Steven M
Biology Department, University of Massachusetts Amherst, 221 Morrill South, 611 North Pleasant Street, Amherst, MA 01003, USA.
Lee Wei-Lih
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Article Info
Journal
Developmental cell
Abbr.
Dev Cell
ISSN
1878-1551
DOI
10.1016/j.devcel.2011.04.011
Published
2011-05-17
Pages
639-51
Language
English
Region
United States
NLM ID
101120028
PMCID
PMC3099244
Subset
IM
Grants
NIGMS NIH HHS · R01 GM076094 · United States
NIGMS NIH HHS · R01 GM076094-05 · United States
NIGMS NIH HHS · 1R01GM076094 · United States
External Links
PubMed source DOI: 10.1016/j.devcel.2011.04.011 PMC Full Text View journal details
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