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PMID: 2154965 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The effect of the gamma-subunit of the cyclic GMP phosphodiesterase of bovine and frog (Rana catesbiana) retinal rod outer segments on the kinetic parameters of the enzyme.

The Biochemical journal ·Vol. 265 ·No. 3 ·1990-02-01 ·Pages 655-8

Whalen MM, Bitensky MW, Takemoto DJ

Abstract

Rod-outer-segment cyclic GMP phosphodiesterase (PDE) (subunit composition alpha beta gamma 2) contains catalytic activity in alpha beta. The gamma-subunits are inhibitors. Removal of the gamma-subunits increases Vmax. without affecting the Km. The inhibitory effect of a single gamma-subunit (alpha beta gamma) on the Vmax. of alpha beta is much greater in bovine than in frog (Rana catesbiana) PDE. Bovine PDE in the alpha beta gamma 2 state has a Vmax. that is 2.6 +/- 0.4% of the Vmax. of alpha beta. The removal of one gamma-subunit to give alpha beta gamma results in a Vmax. 5.2 +/- 1% of that for maximal activity. Frog alpha beta gamma 2 has a Vmax. 10.8 +/- 2%, and alpha beta gamma has a Vmax. 50 +/- 18%, of the Vmax. of alpha beta. These data suggest that a single gamma-subunit can inhibit the catalytic activity of active sites on both alpha- and beta-subunits in bovine, but not in frog, rod-outer-segment PDE.

MeSH Terms
3',5'-Cyclic-GMP Phosphodiesterases/antagonists & inhibitors,metabolism Animals Cattle Kinetics Macromolecular Substances Photoreceptor Cells/enzymology Radioimmunoassay Rana catesbeiana Rod Cell Outer Segment/enzymology
Chemicals
Macromolecular Substances 3',5'-Cyclic-GMP Phosphodiesterases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Whalen M M
Life Sciences Division, Los Alamos National Laboratory, NM 87545.
Bitensky M W
Takemoto D J
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24 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2650655
Published
1990-02-01
Pages
655-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1133684
Subset
IM
Grants
NEI NIH HHS · EYO6816 · United States
External Links
PubMed source DOI: 10.1042/bj2650655 PMC Full Text View journal details
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