Purification of rod outer segment GTP-binding protein subunits and cGMP phosphodiesterase by single-step column chromatography.

3',5'-Cyclic-GMP Phosphodiesterases/isolation & purification,metabolism Animals Bufo marinus Chromatography, Gel/methods Coloring Agents Electrophoresis, Polyacrylamide Gel GTP-Binding Proteins/isolation & purification,metabolism Guanylyl Imidodiphosphate/metabolism Indicators and Reagents Macromolecular Substances Molecular Weight Photoreceptor Cells/metabolism Rod Cell Outer Segment/metabolism Sepharose/analogs & derivatives

Coloring Agents Indicators and Reagents Macromolecular Substances cibacron blue-sepharose Guanylyl Imidodiphosphate Sepharose 3',5'-Cyclic-GMP Phosphodiesterases GTP-Binding Proteins