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PMID: 2153291 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Bovine cone photoreceptor cGMP phosphodiesterase structure deduced from a cDNA clone.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 1 ·1990-01-00 ·Pages 293-7

Li TS, Volpp K, Applebury ML

Abstract

A full-length cDNA clone encoding the alpha' subunit of cGMP phosphodiesterase (PDE) from bovine cone photoreceptors was selected by probing a retinal library with a DNA fragment encoding the catalytic core of the rod cGMP PDE alpha subunit. Identity of the clone was confirmed by comparing its deduced sequence with cone PDE peptide sequences determined by Charbonneau et al. [Charbonneau, H., Prusti, R. K., LeTrong, H., Sonnenburg, W. K., Mullaney, P. J., Walsh, K. A. & Beavo, J. A. (1990) Proc. Natl. Acad. Sci. USA, pp. 288-292]. The cone PDE alpha' and the rod PDE alpha and beta subunits are encoded by distinct genes. cGMP PDE subunits share a common ancestry with cAMP PDEs and cyclic nucleotide-binding proteins. Sequence comparisons predict the presence of a catalytic core and possible secondary sites for noncatalytic cGMP binding. The presence of a C-terminal CAAX (Cys-aliphatic-aliphatic-Xaa) motif suggests the cone enzyme may be posttranslationally modified by proteolysis, methylation, and isoprenylation.

MeSH Terms
3',5'-Cyclic-GMP Phosphodiesterases/genetics,metabolism Amino Acid Sequence Animals Base Sequence Binding Sites Cattle Cloning, Molecular Cyclic GMP/metabolism DNA/genetics,isolation & purification Gene Library Molecular Sequence Data Molecular Weight Nucleic Acid Hybridization Photoreceptor Cells/enzymology RNA, Messenger/genetics Restriction Mapping Sequence Homology, Nucleic Acid
Chemicals
RNA, Messenger DNA 3',5'-Cyclic-GMP Phosphodiesterases Cyclic GMP
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Li T S
Department of Ophthalmology and Visual Sciences, University of Chicago, IL 60637.
Volpp K
Applebury M L
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.1.293
Published
1990-01-00
Pages
293-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC53249
Subset
IM
Grants
NEI NIH HHS · EY04801 · United States
Databases
GENBANK
M29465, M37838
External Links
PubMed source DOI: 10.1073/pnas.87.1.293 PMC Full Text View journal details
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