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PMID: 21455601 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Age-related changes in the function of autophagy in rat kidneys.

Age (Dordrecht, Netherlands) ·Vol. 34 ·No. 2 ·2012-04-00 ·Pages 329-39

Cui J, Bai XY, Shi S, Cui S, Hong Q, Cai G, Chen X

Abstract

Autophagy is a highly regulated intracellular process for the degradation of cytoplasmic components, especially protein aggregates and damaged organelles. It is essential for maintaining healthy cells. Impaired or deficient autophagy is believed to cause or contribute to aging and age-related disease. In this study, we investigated the effects of age on autophagy in the kidneys of 3-, 12-, and 24-month-old Fischer 344 rats. The results revealed that autophagy-related gene (Atg)7 was significantly downregulated in kidneys of increasing age. The protein expression level of the autophagy marker light chain 3/Atg8 exhibited a marked decline in aged kidneys. The levels of p62/SQSTM1 and polyubiquitin aggregates, representing the function of autophagy and proteasomal degradation, increased in older kidneys. The level of 8-hydroxydeoxyguanosine, a marker of mitochondrial DNA oxidative damage, was also increased in older kidneys. Analysis by transmission electron microscope demonstrated swelling and disintegration of cristae in the mitochondria of aged kidneys. These results suggest that autophagic function decreases with age in the kidneys of Fischer 344 rats, and autophagy may mediate the process of kidney aging, leading to the accumulation of damaged mitochondria.

MeSH Terms
8-Hydroxy-2'-Deoxyguanosine Aging/physiology Animals Autophagy/physiology Biomarkers/metabolism Blotting, Western DNA Damage Deoxyguanosine/analogs & derivatives,metabolism Follow-Up Studies Gene Expression Regulation, Developmental Heat-Shock Proteins/biosynthesis,genetics Immunohistochemistry Kidney/metabolism,ultrastructure Male Microscopy, Electron, Transmission Microtubule-Associated Proteins/biosynthesis,genetics Mitochondria/genetics,metabolism Oxidative Stress/physiology RNA/genetics RNA, Mitochondrial Rats Rats, Inbred F344 Real-Time Polymerase Chain Reaction Sequestosome-1 Protein
Chemicals
Biomarkers Heat-Shock Proteins LC3 protein, rat Microtubule-Associated Proteins RNA, Mitochondrial Sequestosome-1 Protein Sqstm1 protein, rat RNA 8-Hydroxy-2'-Deoxyguanosine Deoxyguanosine
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Cui Jing
Department of Nephrology, Chinese PLA Institute, Beijing, China.
Bai Xue-Yuan
Shi Suozhu
Cui Shaoyuan
Hong Quan
Cai Guangyan
Chen Xiangmei
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Article Info
Journal
Age (Dordrecht, Netherlands)
Abbr.
Age (Dordr)
ISSN
1574-4647
DOI
10.1007/s11357-011-9237-1
Published
2012-04-00
Epub
2011-00-01
Pages
329-39
Language
English
Region
Netherlands
NLM ID
101250497
PMCID
PMC3312632
Subset
IM
External Links
PubMed source DOI: 10.1007/s11357-011-9237-1 PMC Full Text
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