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PMID: 2145264 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Translational efficiency of phi X174 lysis gene E is unaffected by upstream translation of the overlapping gene D reading frame.

Journal of bacteriology ·Vol. 172 ·No. 10 ·1990-10-00 ·Pages 5617-23

Bläsi U, Nam K, Lubitz W, Young R

Abstract

The lysis gene E of bacteriophage phi X174 is entirely embedded in gene D. Expression studies of genes D and E in Escherichia coli minicells and lysis times obtained in the presence or absence of D translation showed that the simultaneous expression of gene D does not affect protein E production. Thus, unlike other overlapping gene pairs, gene E expression is independent from the upstream translation of gene D. lacZ fusion studies and primer extension inhibition analysis (toeprinting) revealed an intrinsically weak E ribosome-binding site, which seems to be the major factor determining the low expression rate of the gene and thus proper scheduling of cell lysis.

MeSH Terms
Amino Acid Sequence Bacteriophage phi X 174/genetics Base Sequence DNA, Viral/genetics Escherichia coli/genetics Genes, Viral Lysogeny Molecular Sequence Data Phenotype Plasmids Protein Biosynthesis Viral Proteins/genetics
Chemicals
DNA, Viral Viral Proteins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Bläsi U
Department of Biochemistry and Biophysics, Texas A&M University, College Station 77843.
Nam K
Lubitz W
Young R
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38 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.10.5617-5623.1990
Published
1990-10-00
Pages
5617-23
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC526873
Subset
IM
Grants
NIGMS NIH HHS · R01 GM027099 · United States
NIGMS NIH HHS · GM27099 · United States
External Links
PubMed source DOI: 10.1128/jb.172.10.5617-5623.1990 PMC Full Text View journal details
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