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PMID: 2144902 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of three monoclonal antibodies that recognize the interferon alpha 2 receptor.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 87 ·No. 18 ·1990-09-00 ·Pages 7230-4

Colamonici OR, D'Alessandro F, Diaz MO, Gregory SA, Neckers LM, Nordan R

Abstract

The interferon system plays an important role in the control of viral infections and cell proliferation. These effects are mediated through the interaction of interferons with specific cell surface receptors. We report here the development of monoclonal antibodies against one of the subunits of the interferon alpha receptor. These antibodies detect a 110-kDa protein in surface-labeled cells and in Western blots, and 130- and 210-kDa bands after crosslinking to iodinated interferon alpha 2. No other subunits are disulfide-linked to the 130-kDa subunit or are coprecipitated by these antibodies. Analysis by two-dimensional gel electrophoresis revealed that the pI of this subunit is 3.5-5.0. We suggest that the protein recognized by these monoclonal antibodies be named the alpha subunit of the interferon alpha receptor.

MeSH Terms
Antibodies, Monoclonal/isolation & purification Blotting, Western Burkitt Lymphoma Cell Division/drug effects Cell Line DNA Replication/drug effects Electrophoresis, Gel, Two-Dimensional Electrophoresis, Polyacrylamide Gel Humans Interferon Type I/metabolism Interferon alpha-2 Interferon-alpha/metabolism,pharmacology Macromolecular Substances Molecular Weight Receptors, Immunologic/analysis,immunology Receptors, Interferon Recombinant Proteins
Chemicals
Antibodies, Monoclonal Interferon Type I Interferon alpha-2 Interferon-alpha Macromolecular Substances Receptors, Immunologic Receptors, Interferon Recombinant Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Colamonici O R
Department of Medicine, University of Chicago, IL 60637.
D'Alessandro F
Diaz M O
Gregory S A
Neckers L M
Nordan R
References (27)
27 references, click to expand
  1. High resolution two-dimensional electrophoresis of basic as well as acidic proteins.
    Cell. 1977 Dec;12(4):1133-41 PMID: 23215
  2. High-affinity binding of 125I-labelled mouse interferon to a specific cell surface receptor.
    Nature. 1980 Apr 3;284(5755):459-61 PMID: 6444699
  3. Binding of 125I-labelled human alpha interferon to human lymphoid cells.
    Int J Cancer. 1981 Nov 15;28(5):575-82 PMID: 6171531
  4. Evidence that types I and II interferons have different receptors.
    Nature. 1981 Dec 24;294(5843):768-70 PMID: 6172715
  5. Interferon receptors. Cross-linking of human leukocyte interferon alpha-2 to its receptor on human cells.
    J Biol Chem. 1982 Dec 10;257(23):13884-7 PMID: 6292213
  6. Characterization of an interferon receptor on human lymphoblastoid cells.
    Proc Natl Acad Sci U S A. 1983 Jun;80(11):3269-73 PMID: 6304703
  7. Alpha interferon for induction of remission in hairy-cell leukemia.
    N Engl J Med. 1984 Jan 5;310(1):15-8 PMID: 6689734
  8. Studies of the interferon receptors.
    Pharmacol Ther. 1984;24(2):259-78 PMID: 6205408
  9. Characterization of human beta-interferon-binding sites on human cells.
    J Biol Chem. 1985 Jan 10;260(1):563-7 PMID: 2578128
  10. Time-dependent cytotoxic action of human recombinant alpha-interferon (Ro22-8181) in vitro and the sensitivity of various cultured leukemia and lymphoma cell lines to it.
    Jpn J Clin Oncol. 1985 Mar;15(1):67-75 PMID: 3981816
  11. Regulation of cell proliferation and differentiation by interferons.
    Biochem J. 1985 Mar 1;226(2):345-60 PMID: 2581554
  12. Treatment of hairy cell leukemia with recombinant alpha interferon: II. In vivo down-regulation of alpha interferon receptors on tumor cells.
    Blood. 1986 Mar;67(3):821-6 PMID: 2936410
  13. Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene.
    Blood. 1986 Jun;67(6):1542-9 PMID: 2423157
  14. Differential human interferon alpha receptor expression on proliferating and non-proliferating cells.
    Eur J Biochem. 1986 May 15;157(1):187-93 PMID: 2940085
  15. The interferon receptors.
    CRC Crit Rev Biochem. 1986;21(3):249-75 PMID: 2430756
  16. The treatment of hairy cell leukemia.
    Blood. 1987 Apr;69(4):979-83 PMID: 3493818
  17. Clinical investigation of human alpha interferon in chronic myelogenous leukemia.
    Blood. 1987 May;69(5):1280-8 PMID: 3471281
  18. Interferons and their actions.
    Annu Rev Biochem. 1987;56:727-77 PMID: 2441659
  19. Interferons in the treatment of human neoplasms.
    J Interferon Res. 1987 Oct;7(5):575-81 PMID: 2445856
  20. Therapy of chronic myelogenous leukemia: chemotherapy and interferons.
    Semin Hematol. 1988 Jan;25(1):62-73 PMID: 2450402
  21. IL-2-dependent expansion of CD3+ large granular lymphocytes expressing T cell receptor-gamma delta. Evidence for a functional receptor by anti-CD3 activation of cytolysis.
    J Immunol. 1988 Apr 15;140(8):2527-33 PMID: 2965722
  22. Homozygous deletion of the alpha- and beta 1-interferon genes in human leukemia and derived cell lines.
    Proc Natl Acad Sci U S A. 1988 Jul;85(14):5259-63 PMID: 3134658
  23. Interferon receptors and their role in interferon action.
    Experientia. 1989 Jun 15;45(6):508-13 PMID: 2525482
  24. Interferon receptors.
    Immunol Today. 1988 Dec;9(12):393-400 PMID: 2475121
  25. Deletions of interferon genes in acute lymphoblastic leukemia.
    N Engl J Med. 1990 Jan 11;322(2):77-82 PMID: 2294436
  26. Absence of IFNA and IFNB genes from human malignant glioma cell lines and lack of correlation with cellular sensitivity to interferons.
    Cancer Res. 1990 Jan 15;50(2):278-83 PMID: 2295067
  27. Genetic transfer of a functional human interferon alpha receptor into mouse cells: cloning and expression of its cDNA.
    Cell. 1990 Jan 26;60(2):225-34 PMID: 2153461
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.87.18.7230
Published
1990-09-00
Pages
7230-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC54717
Subset
IM
Grants
NCI NIH HHS · CA42557 · United States
External Links
PubMed source DOI: 10.1073/pnas.87.18.7230 PMC Full Text View journal details
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