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PMID: 21378170 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The histone modifications governing TFF1 transcription mediated by estrogen receptor.

The Journal of biological chemistry ·Vol. 286 ·No. 16 ·2011-04-22 ·Pages 13925-36

Li Y, Sun L, Zhang Y, Wang D, Wang F, Liang J, Gui B, Shang Y

Abstract

Transcription regulation by histone modifications is a major contributing factor to the structural and functional diversity in biology. These modifications are encrypted as histone codes or histone languages and function to establish and maintain heritable epigenetic codes that define the identity and the fate of the cell. Despite recent advances revealing numerous histone modifications associated with transcription regulation, how such modifications dictate the process of transcription is not fully understood. Here we describe spatial and temporal analyses of the histone modifications that are introduced during estrogen receptor α (ERα)-activated transcription. We demonstrated that aborting RNA polymerase II caused a disruption of the histone modifications that are associated with transcription elongation but had a minimal effect on modifications deposited during transcription initiation. We also found that the histone H3S10 phosphorylation mark is catalyzed by mitogen- and stress-activated protein kinase 1 (MSK1) and is recognized by a 14-3-3ζ/14-3-3ε heterodimer through its interaction with H3K4 trimethyltransferase SMYD3 and the p52 subunit of TFIIH. We showed that H3S10 phosphorylation is a prerequisite for H3K4 trimethylation. In addition, we demonstrated that SET8/PR-Set7/KMT5A is required for ERα-regulated transcription and its catalyzed H4K20 monomethylation is implicated in both transcription initiation and elongation. Our experiments provide a relatively comprehensive analysis of histone modifications associated with ERα-regulated transcription and define the biological meaning of several key components of the histone code that governs ERα-regulated transcription.

MeSH Terms
14-3-3 Proteins/metabolism Cell Line, Tumor Chromatin/chemistry DNA/chemistry Estrogen Receptor alpha/metabolism Gene Expression Regulation, Neoplastic Glutathione Transferase/metabolism Histones/chemistry Humans Phosphorylation Promoter Regions, Genetic Protein Structure, Tertiary Ribosomal Protein S6 Kinases, 90-kDa/metabolism Signal Transduction Trefoil Factor-1 Tumor Suppressor Proteins/metabolism
Chemicals
14-3-3 Proteins Chromatin Estrogen Receptor alpha Histones TFF1 protein, human Trefoil Factor-1 Tumor Suppressor Proteins DNA Glutathione Transferase Ribosomal Protein S6 Kinases, 90-kDa mitogen and stress-activated protein kinase 1
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Li Yanyan
Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Biochemistry and Molecular Biology, Peking University Health Science Center, Beijing 100191, China.
Sun Luyang
Zhang Yu
Wang Dandan
Wang Feng
Liang Jing
Gui Bin
Shang Yongfeng
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Article Info
Journal
The Journal of biological chemistry
Abbr.
J Biol Chem
ISSN
1083-351X
DOI
10.1074/jbc.M111.223198
Published
2011-04-22
Epub
2011-00-04
Pages
13925-36
Language
English
Region
United States
NLM ID
2985121R
PMCID
PMC3077593
Subset
IM
External Links
PubMed source DOI: 10.1074/jbc.M111.223198 PMC Full Text View journal details
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