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PMID: 2136831 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Discrete domains of human U6 snRNA required for the assembly of U4/U6 snRNP and splicing complexes.

The EMBO journal ·Vol. 9 ·No. 1 ·1990-01-00 ·Pages 251-5

Bindereif A, Wolff T, Green MR

Abstract

U6 snRNA sequences required for assembly of U4/U6 snRNP and splicing complexes were determined by in vitro reconstitution of snRNPs. Both mutagenesis and chemical modification/interference assays identify a U6 snRNA domain required for U4/U6 snRNP formation. The results support the existence of a U4/U6 snRNA interaction domain previously proposed on the basis of phylogenetic evidence. In addition, two short U6 snRNA regions flanking the U4/U6 interaction domain are essential to assemble the U4/U6 snRNP into splicing complexes. These two regions may represent binding sites for splicing factors or may facilitate the formation of an alternative U6 snRNA secondary structure during spliceosome assembly.

MeSH Terms
Base Sequence Binding Sites Chemical Phenomena Chemistry Immunosorbent Techniques Molecular Sequence Data Molecular Weight Mutation Nucleic Acid Conformation RNA Splicing/genetics RNA, Small Nuclear/genetics Ribonucleoproteins/genetics Ribonucleoproteins, Small Nuclear Structure-Activity Relationship Transcription, Genetic
Chemicals
RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bindereif A
Max-Planck-Institut für Molekulare Genetik, Otto-Warburg-Laboratorium, Berlin, FRG.
Wolff T
Green M R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-01-00
Pages
251-5
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551655
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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