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PMID: 2123826 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

An HlyB-type function is required for expression of the Enterococcus faecalis hemolysin/bacteriocin.

Infection and immunity ·Vol. 58 ·No. 12 ·1990-12-00 ·Pages 3914-23

Gilmore MS, Segarra RA, Booth MC

Abstract

The nucleotide sequence of a 3,422-bp internal restriction fragment from the Enterococcus faecalis pAD1 hemolysin/bacteriocin-encoding region was determined. This fragment was associated with expression of hemolysin/bacteriocin component L and contained a 2,142-bp open reading frame. The inferred amino acid sequence revealed a protein which shared extensive similarity with HlyB of the Escherichia coli alpha-hemolysin operon. The inferred protein, CylB, was observed to be independently expressed in E. coli and capable of complementing an insertion mutation in the cloned hemolysin/bacteriocin operon in trans. Despite the extensive similarity to HlyB, CylB was incapable of complementing an insertion mutation in hlyB. Cytolysin determinants possessing an HlyB-type transport function are widely dispersed throughout gram-negative genera. We believe this to be the first example of an HlyB-type protein encoded within a cytolysin determinant from a gram-positive bacterium.

MeSH Terms
Adenosine Triphosphate/metabolism Amino Acid Sequence Bacteriocins/biosynthesis Base Sequence Cytotoxins/genetics Enterococcus faecalis/genetics Genetic Complementation Test Hemolysin Proteins/genetics Molecular Sequence Data
Chemicals
Bacteriocins Cytotoxins Hemolysin Proteins Adenosine Triphosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gilmore M S
Department of Microbiology and Immunology, College of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City 73190.
Segarra R A
Booth M C
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.12.3914-3923.1990
Published
1990-12-00
Pages
3914-23
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313755
Subset
IM
Grants
NEI NIH HHS · EY 08289 · United States
Databases
GENBANK
M38052
External Links
PubMed source DOI: 10.1128/iai.58.12.3914-3923.1990 PMC Full Text View journal details
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