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PMID: 2123821 Published · ppublish English Journal Article

Generation and characterization of murine antiflagellum monoclonal antibodies that are protective against lethal challenge with Pseudomonas aeruginosa.

Infection and immunity ·Vol. 58 ·No. 12 ·1990-12-00 ·Pages 3819-28

Rosok MJ, Stebbins MR, Connelly K, Lostrom ME, Siadak AW

Abstract

Two murine monoclonal antibodies, IIG5 (IgG3) and IVE8 (IgG2a), that bind to Pseudomonas aeruginosa type a flagella and type b flagella, respectively, were prepared by conventional hybridoma methodology. Specificity of each monoclonal antibody for type a or type b flagella was demonstrated by enzyme-linked immunosorbent assay, indirect immunofluorescence, and immunoblotting. The percentage of P. aeruginosa isolates recognized by each monoclonal antibody was analyzed by enzyme-linked immunosorbent assay. Among a panel of 257 flagellated P. aeruginosa clinical isolates, IIG5 bound to 67.7% of the isolates and IVE8 bound to another 30.7%, for a combined coverage of 98.4%. Inhibition of motility of P. aeruginosa by the monoclonal antibodies was observed in vitro in a soft agar assay and was dose dependent. The protective efficacy of IIG5 and IVE8 was examined in a mouse burn wound sepsis model. The antiflagellum monoclonal antibodies provided specific and significant prophylactic and therapeutic protection against lethal challenge with P. aeruginosa strains.

MeSH Terms
Agglutination Animals Antibodies, Monoclonal/biosynthesis,immunology Female Flagellin/analysis,immunology Fluorescent Antibody Technique Immunoblotting Mice Mice, Inbred BALB C Molecular Weight Movement Pseudomonas Infections/prevention & control Pseudomonas aeruginosa/immunology
Chemicals
Antibodies, Monoclonal Flagellin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rosok M J
Oncogen, Seattle, Washington 98121.
Stebbins M R
Connelly K
Lostrom M E
Siadak A W
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.12.3819-3828.1990
Published
1990-12-00
Pages
3819-28
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313741
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.58.12.3819-3828.1990 PMC Full Text View journal details
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