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PMID: 2427453 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Functionally active monoclonal antibody that recognizes an epitope on the O side chain of Pseudomonas aeruginosa immunotype-1 lipopolysaccharide.

Infection and immunity ·Vol. 53 ·No. 3 ·1986-09-00 ·Pages 656-62

Stoll BJ, Pollack M, Young LS, Koles N, Gascon R, Pier GB

Abstract

A murine monoclonal antibody (MAb) was prepared against Pseudomonas aeruginosa immunotype-1 (It-1) lipopolysaccharide (LPS). The MAb bound It-1 LPS in the enzyme-linked immunosorbent assay and in the immunodiffusion and immunoblotting assays, agglutinated and opsonized It-1 bacteria, and protected against challenge with live It-1 organisms in a murine burn infection model. All of these activities were immunotype specific. Correlation of the opsonic and protective properties of the MAb with its recognition site on the LPS O side chain confirmed that such immunotype-specific determinants are important targets for protective antibodies in Pseudomonas disease. The functional equivalence of this MAb and polyclonal antibodies from hyperimmune plasma underscores the therapeutic potential of single MAbs which recognize critical determinants in the LPS O side chain.

MeSH Terms
Animals Antibodies, Monoclonal/immunology,therapeutic use Enzyme-Linked Immunosorbent Assay Epitopes/analysis Female Lipopolysaccharides/immunology Mice Mice, Inbred BALB C Phagocytosis Pseudomonas Infections/prevention & control Pseudomonas aeruginosa/immunology
Chemicals
Antibodies, Monoclonal Epitopes Lipopolysaccharides
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Stoll B J
Pollack M
Young L S
Koles N
Gascon R
Pier G B
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32 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.53.3.656-662.1986
Published
1986-09-00
Pages
656-62
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260844
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.53.3.656-662.1986 PMC Full Text View journal details
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